trangenic plants (tDNA)

Question 1

what is a common bacteria used to make transgenic plants?

Answer 1

agrobacterium tumefacians as it naturally infects DICOTS and creates a crown gall

rhibozium is better at infecting monocots

Question 2

decribe the TI plasmid

Answer 2

it has left and right border tandem repeats and 3 genes auxin, cytokinin and opine which help to signal replication

we can use the left and right bordrs in biotechnology and virulence region to facilitate movement of tdna into the plant genome

Question 3

what are the requitemetns of a vector

Answer 3

need an origin of replication and a promoter sequence to ensure the gene is transcribed)(expression vector)
Unique cutting site

need a selectable marker to show that the plasmid has been integrated into genome and select for the transformed plant

Question 4

what is the difference between stable and transient transformation?

Answer 4

stable - growing plant from seed to soil, quite slow but you can isolate pure transformed strains

transient - you inject a growing plant with a vector but this is not permanently inherited

Question 5

why are helper plasmids used in transformatin?

Answer 5

a plasmid with both a virulence region and the target gene is very large so we can use 2 plasmid vectors, the main one and the helper one with the vir region

Main one with gene insert, left and right borders and the MCS
We DON’T NEED THE TDNA