dna sequencing and bioinformatics

Question 1

Creating SCAFFOLDS is often required for:
A de novo genome sequencing
B exome sequencing (whole exon)
C genome re-sequencing
D RNA-sequencing

Answer 1

option A - denovo
scaffolds from PacBio or nanopore are useful as they have longer reads than those generated by illumina so they overcome the issue of repeat sequences

but i guess you can also use scaffolds in genome re- sequencing. instead you use the reference genome as the scaffold (so you haven’t actually created scaffold)

Question 2

Which DNA sequencing platform would be most appropriate for analysing the molecules created as part of an RESEQUENCING experiment?
A Illumina.
B Nanopore.
C PacBio.
D Sanger.

Answer 2

illumina as we get short accurate reads and lots of them too. Could use nanopore but would be more expensive