Trace Evidence Flashcards
Describe Locard’s exchange principle
Suggests that every contact leaves a trace
Includes things like fingerprints, blood, hair, clothes fibres, tool marks, scratches, semen etc.
Also tells us that physical evidence cannot be wrong, it can only be missed through human error
Where might you find trace evidence
At Scene
On Clothing
On Victim - and in their injuries
On Suspect - on their clothing or person
On personnel involved in scene or transfer - paramedics,
police, scientists, forensic Nurse, FME, pathologist, PF
Anyone who attends a crime scene can be involved in the transfer of trace evidence - true or false
True
Even without noticing you can contaminate the scene or be contaminated (e.g. leave a hair, pick up a fibre etc.)
Must be aware of all personal at the scene and beyond
This is why hair nets, white suits are used
At it’s simplest, which 3 objects will trace evidence be passed between at a crime scene
Evidence from the scene itself
The body/victim
And the assailant
Doesn’t happen in practice - anyone else in contact with scene may then transfer evidence
Which trace evidence would you look for on clothing
Hairs Fibres Glass fragments Paint - flakes or smears GS residues Vegetation or pollens - outdoor crime scenes
Which clothing stains may be analysed in criminal cases
Blood
Semen - sexual cases
Vomit - analysis of stomach content, especially drugs
Mud / soil - gives a lot of info about location of crime/whether they’ve been moved
How can damage to clothing be useful in criminal cases
Cuts, stabs - sharp force
Tears - blunt force
Location and extent of damage can be evidence
How can pollen analysis be used in criminal cases
Can tell you where someone has been and when they were there
Very specific shapes/sizes that can be analysed down microscope - resitant particles
Different locations will have different plants and therefore different types of pollen - can identify location
Pollen also has both diurnal and seasonal variations which indicates timings
How can paint flakes be analysed
They often have laminated (multi-layered structure) that can be viewed under a microscope
This makes it more unique and allows you to match it to a suspect source
Layer structure can be very specific for identifying cars - can sometimes date a paint chip and match to a specific vehicle (make, model, year)
Is Locard’s principle still relevant to modern forensic medicine
YES
Where are glass fragments often found
Can get tangled in the hair
Combed out by pathologist and sent for analysis
How can glass fragments be analysed
Look at their refractive index, density etc.
These properties can be matched to suspect vehicle/location etc.
Pink stained vomit suggests what
Amitriptyline overdose
Clothing searches are routine at autopsy - true or false
True
Done whether the death is natural or unnatural
Why must you be careful when searching a victims clothes
May have weapons or sharps on them
Particualrly drug abusers - risk of needlestick injury/infections
What is meant by Jigsaw evidence
When you can physically match broken pieces of evidence to the scene/substance
Anything that’s torn or broken can be physically put back together to show they had the same origin
Very powerful evidence
List examples of Jigsaw evidence (physical matching)
Plastic - RTA (number plate etc.)
Metal - from a car or tool, tip of knife (if left in body can be matched to suspect weapon)
Paper or tape when torn
Anything breakable!
How does velocity affect blood splatter
The droplets decrease in size as the velocity increases
Can go from large droplets/pools to a fine mist
The smaller the droplets the less distance they can travel
What can cause a low velocity blood splatter
May be dripping from body, thrown off weapon splashed or spurts of arterial blood
What can cause a medium velocity blood splatter
Assault with a weapon such as a baseball bat
Striking a ‘pool’ of blood with great force
What can cause a high velocity blood splatter
Gunshots
High speed machinery
What would cause a fine mist of blood
A high velocity injury/trauma such as a gunshot wound
What can happen to blood after it has splattered
Can be transferred or smeared onto other objects
How is leuco malachite green used at crime scenes
It is a on-scene test used to indicate the presence of blood
Filter paper has LMG chemicals added and is then touched to a suspect area
If it turns green on contact it means blood is present
Non-destructive chemical test (don’t need to dissolve out the blood)
How can blood splatter distribution be analysed
Can look at the length/width of the stains - if you use this to make a ratio you can determine the angle/height it struck the surface
Will also have a direction to then - can draw lines to show their point of origin
Long and thin blood splatters suggest what
That the blood has struck the surface at a shallow angle
Can be used to estimate location of source
Short and fat blood splatters suggest what
That the blood has struck the surface at a less shallow angle
Can be used to estimate location of source
What is the typical splatter pattern of an arterial bleed
Will be pulsatile - up and down and up and down
Which non-intimate samples can be taken on arrest
Fingerprints, palm prints, other impressions
Photographs - of suspect
Which non-intimate samples can be taken on arrest as long as the inspector gives permission (i.e. reasonable force)
Body hair, other than pubic
Finger or toe-nail clippings or other material from nails
Blood or other body tissues or fluid gained by swabbing or rubbing - not a needle test
Saliva that can be swabbed from the inside of the mouth
Which non-intimate sample requires a warrant
A blood sample
Do police have the power to carry out DNA tests
Yes
Upon detention or arrest, police can carry out a DNA test
Cells scraped/swabbed from inner cheek lining
What are the 6 main benefits of the National DNA database
Early identification of linked cases (serial crimes) Early arrest of offenders Valuable intelligence Early exoneration of innocent suspects Easier identification of bodies Deterrence
What is the purpose of the National DNA Database
Holds DNA profiles from those arrested for charged with, informed they will be reported for, or convicted of a recordable offence (e.g. not a minor offence)
Also holds DNA profiles obtained from crime scenes - allows those arrested to be matched to past crimes
Who manages the National DNA database
the Forensic Science Service on behalf of the police
What happens to stored DNA, prints etc. if the suspect is convicted
They are retained on police files
What happens to stored DNA, prints etc. if the suspect is not convicted
They must be destroyed
What happens to stored DNA, prints etc. if the suspect is given an alternative to prosecution such as a fine or work order
They can be held for up to 2 years
Even 3 if it was a violent or sexual crime
After this it is destroyed
How long can police store DNA, prints etc. from a suspect in a violent or sexual crime
Up to 3 years
Even if they are acquitted or the case dropped
Police can also apply for an extension, to enable retention of DNA information for further periods of up to 2 years at a time
What is required for an intimate sample to be taken
Requires consent from the person and a Dr
Police cannot take them
List examples of intimate samples
Blood - taken via venipuncture Semen Any other tissue fluid Urine Pubic hair Dental impression Swab from body orifice other than the mouth (vagina, anus)
Can an intimate sample ever be taken without consent
Technically yes
Can be taken on the authority of an Inspector from a person in police detention if there are reasonable grounds for suspecting the involvement of the person in a recordable offence and for believing the sample will tend to confirm or disprove his/her involvement
However, most Drs would decline to take it as the GMC and defence unions would not back them
List potential sources of DNA
Blood Tissue Semen - one of the best Saliva -contains cheek cells Hair follicles - not just the shaft Weapon Bullet (exited) Condom Fingernail scrapings - if victim has scratched attacker Bite mark - can swab to recover perpetrator DNA Glass / bottle - drank from or inserted Cigarette - saliva on butt Stamp - if licked will have saliva/cells on it Dandruff Fingerprints Clothing, hat - cells found in these
Which part of the hair can you get DNA from
Only the follicle
Can’t get DNA from shaft only
Why is a condom such a good source of DNA
Will have victims DNA on outside (vaginal or anal cells) and assailant on inside (sperm)
Why is semen such a good source of evidence
Contains loads to DNA specific to individual - head of sperm cells are packed with paternal DNA
Also contains AP and PSA which can be used in analysis
How might you find victim’s DNA on the male suspect following a sexual assault
Vaginal cells containing victim’s DNA rubs off onto penis
This can persist for around 24hrs and even transfer into the perpetrators underwear
What are the main components of semen
Sperm - come from testes, around 20-100 million per ejaculate
Seminal fluid - mixture of fluids from the prostate and seminal vesicles
Forms a whitish fluid with a pH of 7.2-8
How much semen is produced per ejaculate
Around 2-6ml
What happens to sperm motility as time progresses
It decreases
There is rapid progression within 60 minutes of ejaculation
What are the most important constituents of semen in terms of analysis
Spermatozoa (from testes)
Acid Phosphatase (from prostate)
Prostatic Specific Antigen P30 (from prostate)
Choline, Spermine
Describe the appearance of semen immediately after ejaculation
Sticky, jelly-like liquid often forming globules
Usually white in colour, but may appear grey or even yellowish
Why might semen appear pink
Blood in the semen (hematospermia) can cause a pink or reddish colour
Describe the appearance of semen between 5-40 mins after ejaculation
It becomes more watery and liquid before finally drying in
How is semen detected at a crime scene
It will fluoresce under UV light - can scan scene with light
May also look for a dry, crusty stain - more likely than fresh sample at scene
How long do sperm survive post ejaculation
Motile sperm only live up to 4-6 hours in the vaginal canal Intact sperm (with tails) not usually found after 16 hours but have been found up to 72 hours in the GU tract Non motile sperm (no tails) normally found in upper vagina up to 3 days after intercourse, and sometimes up to 6 days or later in the cervix.
How does the pH of the vagina affect sperm motility
Acidic environment of the vagina decreases motile sperm count rapidly
How can sperm be lost
Dilution -by vaginal secretions, saliva
Drainage -under gravity, from vagina to/from anus
Degradation (seminal enzymes, loss of tails)
Defecation - flushes out anal sperm
Victim may wash or drink (dilutes)
How does semen typically drain from the vagina
Most commonly drains from the vagina down to the anus if lying on back (with gravity)
Vaginal contamination from anal semen deposits is rarer but can be seen
Why should samples be taken ASAP in sexual assault cases
Prevents the loss of semen samples