topic two Flashcards
cell surface membrane
- made of lipids and proteins
- regulates movement of substances in and out of the cell/has receptor molecules on it which allow it to respond to hormones.
nucleus
- surrounded by nuclear envelope which contains pores. pores allow RNA to move between cytoplasm.
-chromatin consists of DNA RNA and proteins. nucleolus consists of DNA RNA and proteins too - controls cells activities by controlling DNA transcription/ controls gene expression, protein synthesis and storing DNA.
ribosome production and protein synthesis in nucleolus.
mitochondria
- oval shaped structure with outer and inner membranes. inner is folded to form cristae and inside is called matrix which contains enzymes for respiration
- site of aerobic respiration, releases energy
chloroplast
- have a double membrane that surrounds the stoma. inside the stoma there is fluid filled sacs called thylakoids.
- site of photosynthesis; light independent reactions in sacs, light dependent reactions in stoma
golgi apparatus
- composed of small vesicles, a membrane bound structure filled with fluid
- processes and packages lipids & makes lysosomes
lysosome
- surrounded by a membrane with no clear internal structure.
- contains lysozyme, kept separate from cytoplasm by surrounding membrane, used to digest invading cells
ribosome
- floats free in cytoplasm/or attached to RER, made of proteins and RNA
- site where proteins are made
rough endoplasmic reticulum
- system of membranes enclosing a fluid filled space, surface covered with ribosomes
- folds/processes proteins made at ribosome
smooth endoplasmic reticulum
- no ribosomes, but similar to RER
- synthesises and processes lipids
cell wall
- rigid, surrounds membrane. cellulose in plants and chitin in fungi
- supports cells, protection against pathogens
vacuole
- membrane bound (called the tonoplast), contains cell sap (solution of sugars and salts)
-maintains osmotic pressure, stops plants wilting, stores unwanted chemicals
differences between prokaryotic compared to eukaryotic
- cytoplasm has-no bound organelles
- no nucleus, circular DNA instead
- plasmids
- capsule surrounding the cell
- flagellum
structure of virus particles
- genetic material surrounded by capsid
- lipid envelope with attachment proteins
formula for magnification
image = actual size x mag
how high can a light microscope measure resolution
0.2 micro metres
why can light microscopes only measure a small amount of resolution
the microscope cant produce the image of an object that is smaller than the wavelength of light
how high can an electron microscope measure resolution to
0.1 nm
what are the two types of electron microscopes
- scanning electron microscope
- transmission electron microscope
how do TEMs work
a beam of electrons passes through a thin piece of the specimen.
areas that absorb electrons appear darker
how do SEMs work
a beam of electrons passes across the surface and scatters.
this builds up a 3D image
limitations of SEMs
- lower resolution
- specimens have to be dead
strength of SEMs
- can produce 3D images
limitations of TEMs
- samples need lots of prep
- very expensive
- cant produce 3D images
strengths of TEMs
- high resolution
specimens can be alive
what is cell fractionation
separating cells and organelles to study them in detail
explain homogenisation
- cells are blended in a homogensier to form a fluid called homogenate.
- this is added to a centrifuge
- the heaviest organelles are forced to the bottom where a sediment forms (lowest speed)
- the fluid at the top is called the supernatant, this is removed and added to a different tube
- the supernatant is then spun at higher speeds to force the smaller organelles
- the last organelle to be forced to the bottom is ribsomes.
why is the solution ice cold
to stop the organisms from breaking down organelles
why is a buffer solution used
to maintain pH