topic four Flashcards
what do DNA and RNA do (comparison)
- DNA holds genetic info whereas RNA transfers the genetic info from DNA to ribosomes.
what is the genetic code
the order of bases on DNA
what does each triplet of bases code for
a particular amino acid (known as a codon)
what is a gene
a sequence of DNA which codes for either a polypeptide or functional RNA
what is a locus
the location of a gene
what are the non-coding sections of DNA called
introns
what are the coding regions called
exons
how many possible combinations of the triplets are there
64 (4^3) - means each amino acid is represented by more than one triplet
features of the genetic code
- non overlapping
- degenerate
- universal
what does non overlapping mean
each triplet is only read once and odes not share any bases
what does degenerate mean
there are more possible combinations of triplets than there are amino acids (64 comb, 20 AA)
what does universal mean
same specific base triplets code for the same amino acids in all living things
DNA in eukaryotes
- long
- linear
- has introns
- has histones (proteins)
DNA in prokaryotes
- short
- circular
- no introns
- no histones (proteins)
how many chromosomes does a human have
46 (23 pairs) - arranged into homologous pairs
what is a homologous pair
consists of 2 chromosomes that carry the same gene, but have a different allele (therefore not identical)
which chromosome determines the sex of an individual
23
what is an allele
an alternative form of a gene
what are the two stages of protein synthesis
- transcription
- translation
where does transcription occur and what does it involve
- occurs in nucleus
- involves DNA and mRNA
where does translation occur and what does it involve
- occurs at ribosome
- involves mRNA and tRNA
mRNA summary
- made during transcription
- carries genetic code from DNA to ribosomes
- single polynucleotide strand
tRNA summary
- involved in translation
- carries amino acids to ribosomes
- single polynucleotide strand
- H bonds between base pairs
- every molecule has an anitocodon
- has amino acid binding site at the other end
transcription
1, an enzyme causes hydrogen bonds between bases to break. DNA uncoils and separates the two strands
2. one of the strands is used as a template to make the mRNA molecule (template called antisense strand)
3. free nucleotides line up, joined by phosphodiester bonds, forming a molecule of mRNA. when stop codon is reached, this ends. as RNA polymerase moves away, DNA reforms
4. in eukaryotes, DNA is then spliced to remove introns. mRNA then moves out of nucleus through a pore and attaches to a ribosome in cytoplasm
translation
- mRNA attaches to ribosome and tRNA collects amino acids from cytoplasm and carries them to ribosome.
- tRNA attaches itself to mRNA by complementary base pairing
- amino acids attach to two tRNA molecules by a peptide bond and tRNA molecules then detatch themselves from amino acids, leaving them behind
- process is repeated to form a polypeptide chain until a stop codon is reached. protein synthesis ends.
what is a mutation
a change in the base sequence of DNA
what are the three types of mutation called
- addition
- substitution
- deletion