topic 8 gene expression/gene tech

Question 1

Explain how determining the genome of a pathogen could allow vaccines to
be developed {2}

Answer 1

.Could identify the pathogen’s proteome

●So could identify potential antigens (proteins that stimulate an immune response) to use in the vaccine

Question 2

DEFine Genome {1}

Answer 2

complete set of genes in a cell

Question 3

define proteome {1}

Answer 3

full range of protiens that a cell can produce

Question 4

Explain why the genome cannot be directly translated into the proteome in
complex organisms {2}

Answer 4

. Presence of non-coding DNA (eg. introns within genes do not code for polypeptides)
● Presence of regulatory genes (which regulate expression of other genes, eg. by coding for miRNA)

Question 5

Explain how a mutation of a tumour suppressor gene can result in the formation of a tumour (2)

Answer 5

.gene inactivated/not able to control/slow down cell division

.Rate of cell division too fast/out of control

Question 6

Explain why the same restriction endonucleases were used on each DNA sample (2

Answer 6

.Cut DNA at same base sequence

.So get fragments with required gene