Topic 7: Genomics Part 2 Flashcards

1
Q

What are transposable elements

A

Aka transposons. Sequences that can move about the genome. They are of viral origin

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2
Q

How repetitive are TEs

A

Moderately

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3
Q

Features of TEs

A
  • transposase (enzyme responsible for excising, relocating)
  • direct repeats
  • inverted repeats
  • reverse transcriptase
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4
Q

What is transposition

A

Movement of the transposons

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5
Q

Three steps of transposition, performed by tranposase

A
  1. Staggered breaks are made in the target DNA site
  2. Transposable element is joined to single stranded ends of the target
  3. DNA is replicated at the single stranded gaps
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6
Q

What are direct repeats

A

Not part of the TE, but are generated by transposition

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7
Q

What are inverted repeats

A

Are part of the TE, direct the tranposase

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8
Q

Two main classes of TE

A

Class 1 - retrotransposons: work through an RNA intermediate (copy/paste)
Class 2 - DNA transposons: cut/paste mechanism, non-replicative

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9
Q

Describe class 1 transposable elements

A

Retrotransposons
Copy/paste mechanism (replicative)
Encode a reverse transcriptase that can create and integrate cDNAs into the genome

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10
Q

Examples of class 1 TE

A

SINE and LINE short and long interspersed elements
Alu element: 300 bases long

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11
Q

Slide 8

A

Retrotransposons

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12
Q

What is metagenomics? Examples

A

Sequencing all DNA from an environment to find out which species or genes are present
Ex soil biome, gut microbiome, lake/ocean sample

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13
Q

Why do metagenomics?

A

Most microorganisms are unculturable in lab setting, may be crucial to understanding ecosystem

Allows sequencing of all the genomes present in a sample

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14
Q

Metagenomics can determine..

A

species present (biodiversity) or genes present (biome function)

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15
Q

What is genome wide association mapping

A

Involves detecting statistical associations between single nucleotide polymorphism markers and phenotypes (genotype-phenotype association) using a large sample of unrelated individuals

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16
Q

Example of genome-wide association mapping

A

Collect a large dataset of mapped SNPs in
1. 1000 ppl with particular type of cancer
2. 1000 people without cancer
^ phenotype

Are there SNP alleles that most of the ppl with cancer have but few of the ppl without cancer have?

17
Q

Why use genome-wide association mapping? (4)

A
  1. Some species cannot be mutagenized and studied in lab (humans)
  2. some species can not be crossed and studied in lab (whale)
  3. some phenotypes involve many genes (no good/clear phenotypes when knocked out)
  4. many scientists interested in natural variation/mutations and how they evolve
18
Q

Slides 13-15**

A

Genome-wide association mapping

19
Q

Who got the nobel prize for discovering transposable elements

A

Barbara McClintock