Topic 7 Flashcards
Explain tendons
-Non-elastic tissue which connects muscles to bone
Explain ligaments
-Elastic tissue which joins bone together
Explain joints
-Area where 2 bones are attached
-Allows body parts to move
-Made of fibrous connective tissue plus cartilage
Explain antagonistic muscle pairs
-Pair of muscles which pull is opposite directions
-One contracts as the other relaxes
-Extensors straighten joint
-Flexors bend the join
What is a sarcolemma, sarcoplasm
-Sarcolemma: Muscle Cell Membrane, has inwards folds named tubules which help spread electrical impulses
-Sarccoplasm: Muscle Cytoplasm containing Ca2+ ions
Explain Skeletal Muscle
-Made of large bundles of muscle fibres
-Sarcolemma which has inward folds some stick into sarcoplasm named tubules
-Tubules help spread electrical impulses throughout sarcoplasm
-Sarcoplasmic reticulum runs through sarcoplasm, stores and releaeses Ca ions needed for muscle contraction
-Lots of mitrochondria, for ATP, for muscle contraction
-Lots of long cylindrical organelles named myofibrils, made of specialised proteins for muscle contraction
Explain Myofibrils
-Darker bands are thick myosin filament
-Lighter bands are thin actin filaments
-Made of many short units named sarcomeres
Explain Myofibril bands
-A: Dark bands contain thick myosin overlapping with thin actin
-I: Light bands contain thin actin filament only
-Z: Ends of the sarcomere are marked with a Z Line
-M: M line is the middle of a myosin filament
-H: Contains myosin filament only
Explain Sliding filament theory
-Explains muscle contraction
-Myofilaments slide over one another to make sarcomeres contract
-Myofilaments do not contract, their length stays fixed
-Simultaenous contraction of many sarcomeres means myofibrils and muscle fibres contract
-Sarcomeres return to their original length when muscle is relaxed
Explain Myofilaments
-Both have globular heads capable of back and fourth movement
-Myosin head has a binding site for actin and ATP
-Actin filaments have binding site for myosin heads, actin-myosin binding site
-Tropomyosin and troponin and found inbetween actin filaments
-They are attached to eachother to help myofilaments move past eachother
Explain tropomyosin
-In resting muscle, actin-myosin binding site is blocked by tropomyosin held by troponin
-This prevents myofilaments sliding past each other as the head is unable to bind to actin-myosin bridge (actin filament)
Explain how muscle contraction is triggered by an action potential
-A.P from motor neurone stimulates muscle cell, depolarising the sarcolemma
-Depolarisation spreads to the tubules to sarcoplasmic reticulum
-Sarco.Retic releases Ca2+ ions into the sarcoplasm which bind to troponin, causing a change of its shape
-Tropomyosin is pulled out of the actin-myosin binding site on actin
-Exposed binding site allows myosin head to bind
-Bond is formed, actin-myosin cross bridge,
-myosin head pulling the actin filament in a rowing action
-Ca2+ also activates ATPase… next card
Explain how ATP plays a role in the actin filament (actin-myosin binding site)
-ATP provides energy for the myosin head to move to the binding site
-Ca2+ activated ATPase which breaks down ATP to provide the energy
-Energy released then moves the myosin head pulling the actin filament in a rowing action
Explain ATP breaking cross bridges
-ATP from Ca presence provides energy to break the actin-myosin cross bridge
-This means the myosin head detaches after its movement
-Myosin head reattaches to a different binding site further up the filament
-A new actin myosin cross bridge is formed and the cycle repeats for as long as calcium binds to troponin
Explain what happens when excitation of muscle stops
-Muscle is no longer stimulated, Ca2+ ions are no longer released
-Ca2+ ions on troponin leave their binding site and are actively transported back into Sarc.Retic
-Troponin returns to its original shape pulling tropomyosin molecules with them which now block the actin-myosin binding site
-This means muscle no longer contracts as myosin head does not bind causing a rowing action
Explain slow twitch muscles
-Muscles that contract slowly e.g posture ones - in your back
-Great for endurance activities e.g long running
-Can work for long without fatigue
-Energy is released slowly via aer.resp, mitochondria and blood vessels supply oxygen
-Reddish in colour due to high myoglobin amount - myoglobin stored oxygen
Explain fast twitch muscles
-Muscles that contract rapidly
-Muscles used for fast movement e.g in eyes or legs
-Great for short burst of speed and power
-Fatigues quick
-Energy released quickly via aer.resp, using glycogen (stored glucose)
-Fewer mitochondria and blood vessels
-Whitish in coour due to less myoglobin count therefore less O2 stored
Explain aerobic respiration
-Process of large energy release by splitting glucose into CO2 and H2, which forms H2O
-Energy released is used to phosphorylate ADP to ATP, to provide energy for biological processes
-4 stages: glycolysis, link reaction, krebs cycle, oxidative phosphorylation
-Products from first 3 stages are used in 4th stage to produce many ATP
-Coenzymes (NAD, FAD) are used to transfer H from one molecule to the next
Explain glycolysis
-Occurs in cytoplasm
-Glucose (6C) is phosphorylated using 2x ATP, 2x Phos added to sides of glucose
-Splitting of 1x glucose (6C) into 2x triose phosphate (3C), 1Phos on side of 3C
-Phosphorylation occurs again, 2x Phos on both sides of both triose phosphate
-Triose Phosphate is oxidised to form 2x Pyruvate (3C) (phosphate is removed from molecule)
-2x NAD + H -> reduced NAD and 2x ADP +Pi -> ATP
-Net: 2 ATP produced, 2x Pyruvate + H2O, 2x NADH + 2H
Explain link reaction
-Occurs in matrix of mitrochondria, active transport via transport protein across mitochondrial envelope, using H ion.
-Occurs twice for every glucose molecule since 2x Pyruvate is made
-Decarboxylation and dehydrogenation of 3C is catalysed by pyruvate dehydrogenase
-Dehydrogenation occurs: 1x NAD -> Reduced NAD
-Pyruvate is decarboxylated from 3C -> 2C (Acetyl), lost carbon is in CO2 form
-Acetyl combines with coenzyme A to form acetyl-CoA
-Net: 2x acetylCoA goes into krebs, 2x CO2 is waste
-Net: 2x Reduced NAD is formed
Explain krebs cycle
-Occurs in matrix of mitrochondria and occurs twice (since 2 acetylcoa)
-Coenzyme A released from Acetyl
-Acetyl (2C) reacts with acetate (4C) to form citrate (6C)
-Dehydrogenation occurs on Citrate (6C), producing reduced NAD
-Decarboxylation occurs, CO2 wasted, citrate becomes 5C
-Dehydrogenation occurs on 5C, producing reduced NAD
-Decarboxylation occurs again on 5C, CO2 wasted, becomes 4C
-substrate level phosphorylation occurs, 1x ADP + Phos -> ATP
-4C combines with CoA temporarily
-Dehydrogenation occurs twice on 4C, reduced FAD and reduced NAD produced
-A new 4C is produced (oxaloacetate is regenerated allowing cycle to repeat)
-Net: 3x reduced NAD, 1x reduced FAD, 2x CO2, 1x ATP (ONE ACETYL)
Explain oxidative phosphorylation
-Inner mitochondria membrane is an ETC and ATP synthase
-Reduced NAD and reduced FAD transfers 2 high energy electrons into the ETC becoming unreduced
-Electrons move down the ETC, reducing and oxisiding ETC proteins
-Electron moves and energy is lost which is taken by ETC proteins to pump protons from matrix into intermembrane space - proton conc gradient increases here
-Protons move down the gradient into the ATP synthase. The energy from movement is used to create ATP whilst pushing protons into matrix. - This is chemiosmosis
-In the matrix, at end of ETC, electrons combine with 1/2oxygen and 2H to produce H2O
-Oxygen is named the final electron acceptor
Explain anaerobic respiration
-Respiration without oxygen
-Only glycolysis occurs
-Lactate fermentation occurs in animals and produces lactate
-After glycolysis occurs, reduced NAD is converted into NAD and lactic acid is produce
-Glycolysis produces a net of 2 ATP which can keep biological processes going
-Lactic acids needs to be broken down by
-Converting it back to pyruvate to go into krebs cycle
-Liver cells converting it into glucose to be respired or stored
Explain cardiac muscle
-Cardiac muscle controls the regular beating of the heart
-It is myogenic meaning it can contract and relax without signals via neurones
-SAN in the RA sets the rythm of the heartbeat by sending out waves of electrical activity to atrial walls
-This causes RA and LA to contract in sync
-A band of non conducting collagen tissue prevents electrical wave passing into ventricles from atria
-AVN is responsible for passing waves of electricity onto the bundle of his
-Bundle of his is a group of muscle fibres which conduct electrical waves to finer muscle fibres in the ventricular walls named purkyne fibres
-Purkyne fibres carry electric wave to muscular walls of ventricles to contract in sync