Topic 5: Energy Transfers Flashcards
What does photoautotrophic mean?
Organisms capable of synthesising their own food from inorganic ions using light as an energy source
Name 5 uses of ATP
- Nerve impulses and muscle contraction
- Activation of molecules
- Synthesis of molecules
- Active transport
- Cell division
TRUE OR FALSE?
Phagocytosis requires ATP
TRUE
What are the 3 main components of ATP?
- An adenine nitrogenous base
- A phosphate group
- A ribose 5C sugar
What is the equation for the synthesis of ATP?
ADP + Pi + energy -> ATP
What enzyme catalyses the formation of ATP?
ATP synthase
What type of reaction of ATP synthesis?
A condensation reaction
What is the reaction for the release of energy from ATP?
ATP + H2O -> ADP + Pi + Energy
When ATP is synthesised, is energy used?
Yes
What enzyme catalyses the conversion of ATP to ADP +Pi?
ATP hyrolase
What type of reaction is ATP -> ADP + Pi?
Hydrolysis
Why is ATP used as an intermediate energy source instead of glucose?
- ATP hydrolysis releases less energy so it is more manageable
- Only a single reaction, so quicker
- An immediate source of energy
What are coenzymes?
Molecules that enzymes need to function
Give 3 coenzymes used in respiration.
- NAD
- FAD
- Coenzyme A
What coenzyme is used in photosynthesis?
NADP
What does OIL RIG stand for?
Oxidation Is Loss
Reduction Is Gain
(of electrons)
What is oxidation?
- Gaining oxygen
- Loosing Hydrogen
- LOSS OF ELECTRONS
What is reduction?
- Gaining hydrogen
- Lossing oxygen
- GAIN OF ELECTRONS
Which of oxidation and reduction causes energy to be released?
Oxidation = Energy released
Reduction = Energy taken in
Oxidation and reduction are said to be _ reactions?
Oxidation and reduction are LINKED REACTIONS
As one molecule becomes oxidised, another becomes reduced
LEAF ADAPTATIONS FOR PHOTOSYNTHESIS
Why do leaves have a high surface area?
LEAF ADAPTATIONS FOR PHOTOSYNTHESIS
To absorb light
LEAF ADAPTATIONS FOR PHOTOSYNTHESIS
Why do leaves have a transparent cuticle and epidermis?
LEAF ADAPTATIONS FOR PHOTOSYNTHESIS
Allows light to reach chloroplasts in the palisade mesophyll cells beneath for the light dependent reaction
LEAF ADAPTATIONS FOR PHOTOSYNTHESIS
Why are leaves thin?
LEAF ADAPTATIONS FOR PHOTOSYNTHESIS
Offers a short diffusion pathway for oxygen through stomata
LEAF ADAPTATIONS FOR PHOTOSYNTHESIS]
Why do leaves have lots of stomata?
LEAF ADAPTATIONS FOR PHOTOSYNTHESIS
To allow gas exchange and water pressure gradient in plants
LEAF ADAPTATIONS FOR PHOTOSYNTHESIS
Why are there large spaces between spongy mesophyll cells?
LEAF ADAPTATIONS FOR PHOTOSYNTHESIS
Allows for rapid diffusion of CO2 and O2
What is the function of xylem with regards to photosynthsis?
The xylem supplies the leaf cells with water
Water is a reactant in photosynthesis - involved in photolysis
What is the function of phloem with regards to photosynthsis?
The phloem carries away the products of photosynthesis
Thylakoids (in chloroplasts) have a large surface area. Why is this useful for photosynthesis?
- Large SA for pigments
- More electron carriers for the light dependent reaction
Where does the light dependent reaction of photosynthesis take place?
In the thylakoid membrane
What are grana in chloroplasts made up of?
Thylakoids
Where does the light independent reaction of photosynthesis take place?
In the stroma of chloroplasts
What are photosystems?
- Groups of pigments (chlorophyll) held in thylakoid membranes
- Maximises the absorption of light energy
- Enables the light-dependent reaction of photosynthesis to occur
What is the role of ATP synthase channels in thylakoid membranes (chloroplasts)?
- Allow protons to move out of the thylakoid lumen down the electrochemical gradient
- The movement through the ATP synthase channels causes the synthesis of ATP by the phosphorylation of ADP
What is the overall equation for photosynthesis?
6CO2 + 6H2O -> C6H12O6 + 6O2
What are the two main stages of photosynthesis?
- Light dependent reaction
- Light independent reaction
How do plants absorb light from multiple wavelengths?
Many types of pigment in photosystems in thylakoid membranes
Which photosystem is first used in the light dependent reaction of photosynthesis?
PSII
What happens to a chlorophyll molecule as it gains light energy?
Electrons gain energy and move to higher energy levels
What is it called when chlorophyll becomes oxidised?
Photoionisation
electron moves to higher energy levels along electron transport chain
Where is the energy released from the photoionisation of chlorophyll in photosystems used?
The energy is conserved in the production of ATP and reduced NADP
What 2 products of the light dependent reaction are used in the light independent reaction of photosynthesis?
- ATP
2. Reduced NADP
What are the 7 steps of the light dependent reaction of photosynthesis?
- PSII absorbs light energy
- Electrons are excited and pass along electron carriers to PSI (photoionsiation)
- Some light energy used in the photolysis of water, releasing electrons, protons and oxygen
- Energy released by electrons as they pass between electron carriers causes protons to be actively transported into the thylakoid lumen (from the stroma)
- At the same time as in stage 1, light is absorbed in PSI
- Electrons excited at PSII travel along electron carriers and join with NADP and H+ ions to produce reduced NADP
- Photolysis of water and H+ ion active transport creates a proton gradient (higher concentration in the thylakoid lumen compared to stroma)
- Protons move through ATP synthase channel from high concentration in thylakoid lumen to the stroma by facilitated diffusion
What is the photolysis of water?
The use of some light energy to split water into electrons, protons and oxygen
What are the electrons released in the photolysis of water used for?
The electrons replace the electrons released by PSII in photoionisation
What happens as electrons pass between electron carriers in the light dependent reaction of photosynthesis and in oxidative phosphorylation in respiration?
- Energy is released
- Allows the active transport of H+ ions
- Sets up H+ ion gradient
Why do protons have to travel through a protein in light dependant reaction of photosynthesis and in oxidative phosphorylation of respiration?
H+ ions are (+vely) charged so cannot pass through phospholipid bilayers
How are leaves arranged to absorb maximum light?
There is minimal overlapping so few leaves are shadowed by another
What happens to the oxygen released from the photolysis of water? (2 points)
- Used for respiration
2. Diffuses out of leaf as a waste product
How is ATP produced by the movement of H+ ions through ATP synthase channels?
- Protons passing through cause ATP synthase enzyme to change shape
- Catalyses the synthesis of ATP (ADP + Pi)
What is the movement of H+ ions through ATP synthase also called?
Chemiosmosis
In what part of photosynthesis is water used?
In the light-dependent reaction
In what part of photosynthesis is CO2 used?
In the light independent reaction
How does CO2 used in the light independent reaction enter the chloroplast?
- Diffuses from atmosphere into leaf via stomata
- Diffuses through water surrounding mesophyll cells
- Diffuses through cell surface membrane, cytoplasm + chloroplast membranes
- Enters the stroma
What are the 8 steps of the Kalvin Cycle?
- CO2 combines with RuBP (5C) and enzyme rubisco
- Forms 2x GP (3C)
- ATP hydrolysed into ADP + Pi
- Reduced NADP to NADP
- 2x TP (3C)
- Useful organic substances produced
- Several enzyme-controlled reactions and ATP hydrolysis
- RuBP (5C)
Restarts!
How many cycles of the Kalvin cycle are required to produce glucose?
6
What product of the light dependent reaction is used to reduce GP?
Reduced NADP
Energy provided by the hydrolysis of ATP
After reduced NADP becomes NADP in the Kalvin Cycle what happens to the NADP?
- Returns to the light-dependent reaction
- Picks up more hydrogen to allow further light dependent reactions to take place
What useful “organic substances” does the Kalvin Cycle produce?
- Hexose sugars
- Fatty acids
- Amino acids
(the building blocks for life)
Why is it important that chloroplasts contain DNA and ribosomes?
Allows quick and easy manufacture the proteins involved in the light dependent reaction
Why is it important that thylakoid membranes are selectively permeable?
Allows the proton gradient to be established in the light dependent reaction of photosynthsis
Would the light independent reaction occur in the absence of light?
In theory yes, however it requires reduced NADP and ATP for the Kalvin cycle to work.
Reduced NADP and ATP are both products of the light dependent reaction, so the light dependent reaction will quickly stop without them
In which two steps does ATP from the light dependent reaction provide energy in the Kalvin cycle?
- Reduction of GP to TP
2. Regeneration of RuBP
What are the 3 main limiting factors that affect the rate of photosynthesis?
- CO2 concentration
- Light intensity (and wavelength)
- Temperature
What is the law of limiting factors?
At any given moment, the rate of photosynthesis is limited by the factor that is at its least favourable value
How can farming practices overcome some limiting factors?
Greenhouses used
- Artificial light to increase ‘daylight hours’
- Artificial heating
- More CO2 added
Is the rate of photosynthesis affected by all the factors involved?
No. It is affected by the factor with the least favourable value
Why are selectively permeable membranes in mitochondria important for respiration?
Allows pyruvate in, but glucose can’t
Why is the large SA created by the mitochondrial cristae important for respiration?
Allows for many electron carriers on the inner mitochondrial membrane
What is used as an immediate energy source by cells?
ATP
How is ATP formed?
ATP is formed from the energy released by the hydrolysis of glucose in cellular respiration (forms ATP from ADP + Pi)
What are the two types of cellular respiration?
Aerobic and anaerobic respiration
Under what conditions does aerobic respiration occur?
When oxygen is present
Under what conditions does anaerobic respiration occur?
When oxygen is absent
What is the simple equation for aerobic respiration?
C6H12O6 + 6O2 -> 6CO2 + 6H2O
ADP + Pi -> ATP in the conversion
What is the simple equation for anaerobic respiration in animals?
Glucose -> Lactate
What is the simple equation for anaerobic respiration in plants and yeast?
Glucose -> Ethanol + CO2
What are the 4 stages of aerobic respiration?
- Glycolysis
- Link reaction
- Kreb’s Cycle
- Oxidative phosphorylation
What stage of respiration is involved in both aerobic and anaerobic respiration?
Glycolysis
What basically happens in glucolysis? (respiration)
Glucose (6C) is broken down into 2 Pyruvate molecules (3C)
Where does Glycolysis take place? (respiration)
In the cell cytoplasm
What type of sugar is glucose?
A hexose sugar
Of anaerobic and aerobic respiration, which one produces more ATP?
Aerobic respiration
Which part of respiration is the ONLY part to USE ATP?
Phosphorylation of glucose in glycolysis
Outline what happens in glycolysis (respiration)
- Glucose is phosphoylated to form glucose phosphate
(2x ATP used) - Phosphorylated glucose splits into two TP
- TP is oxidised and NAD is reduced as hydrogen is transferred (oxidation and reduction are liked reactions)
- 4x ATP is synthesised
- TP converts into pyruvate
What is NAD?
A coenzyme (a hydrogen carrier)
What is activation energy?
The energy required to bring about a reaction (lowered by enzymes)
What are the products of glycolysis for every glucose used(resp.)?
2x pyruvate
2x Reduced NAD
2x ATP (net, 2 used, 4 produced)
What is the reduced NAD from glycolysis used for?
The production of ATP in oxidative phosphorylation