Topic 5 and 4- Health And Disease- Development Of Medicane Flashcards
What is a restriction enzyme?
Enzymes that recognise specific sequences of DNA and cut the DNA at these points
What is left after the restriction enzyme cuts the DNA?
Sticky ends
What are ligase enzymes?
Enzymes used to join two process of DNA together at their sticky ends.
What are the bits of DNA stuck together called?
Recombinant DNA
What is a vector in biology?
Something that is used to transfer DNA into a cell
What are the two types of vectors?
Plasmids and viruses
What are plasmids?
Small circular molecules of DNA that can be transferred between bacteria
What are viruses?
They insert DNA into the organisms they infect
What is the first stage of genetic engineering?
The dna you want to insert is cut with a restriction enzyme. The vector dna is also cut open using the same restriction enzyme
What a the second stage of genetic engineering?
The vector dna and the dna that you’re inserting are left with sticky ends and are mixed with ligaments enzymes
What is the third stage of genetic engineering?
The ligase join the prices of DNA together to make recombinant DNA
What is the 4th stage of genetic engineering?
The recombinant dna is inserted into other cells eg bacteria. These cells can now use the gene to make the protein you want
What is the spread of a communicable disease called?
Transmission
What is the name of the molecules that pathogens have
Antigens
What is the spread of communicable disease called?
Transmission