topic 5 Flashcards
structure of chloroplasts
-thylakoid membrane- folded membranes which contain chlorophyll and electron carrier proteins embedded within these membranes. Involved in the LDR.
-stroma- fluid centre which contains enzymes involved in the LIR
-inner and outer membrane- control what can enter and leave the organelle
where does the LDR occur and what is its purpose
on the thylakoid membranes
light energy and water are used to create ATP and reduced NADP which are needed for the LIR
what are the 4 key stages of LDR
photoionisation of chlorophyll
production of ATP and reduced NADP
chemiosmosis
photolysis
describe photolysis
light energy is absorbed by chlorophyll and splits water into oxygen, H+ and e-
H2O –> 1/2O2 + 2e- + 2H+
the H+ is picked up by NADP to form NADPH and is used in the LIR
the e-s are passed along a chain of electrons carrier proteins
the oxygen is either used for respiration or diffuses out of the leaf through the stomat
describe photoionisation of chlorophyll
light energy is absorbed by the chlorophyll and this results in electrons becoming excited and raising up an energy level to leave chlorophyll
some of the energy from the released electrons is used to make ATP and reduced NADP in chemiosmosis
describe chemiosmosis
the electrons that gained energy and left the chlorophyll move along a series of proteins embedded within the thylakoid membrane
as they move along, they release energy and some of this is used to pump the protons across chloroplast membranes
an electrochemical gradient is created-high in thylakoid space and low in stroma so the protons diffuse across the membrane through the enzyme ATP synthase which results in the production of ATP
the protons combine with the co-enzyme NADP to become reduced NADP
where does the LIR/ calvin cycle occur
in the stroma
contains the enzyme rubisco which catalyses this reaction
what are the products and reactants of the calvin cycle
uses CO2, reduced NADP and ATP to produce a hexose sugar
describe the LIR/ calvin cycle
-CO2 reacts with RuBP to form 2 molecules of GP, a 3 carbon compound. this reaction is catalysed by rubisco
-GP is reduced to triose phosphate using energy from ATP and by accepting a H from reduced NADP
-one of the carbons from triose phosphate leaves the cycle each turn to be converted into useful organic substances
-the rest of the molecule is used to regenerate RuBP with energy from ATP
-glucose is the product and can join to form di/polysaccharides or can be converted into glycerol and combine with fatty acids to make lipids for the plant
what are the limiting factors for photosynthesis
temperature
CO2 concentration
light intensity
what are the limiting factors for photosynthesis
temperature
CO2 concentration
light intensity
what are common agricultural techniques to remove limiting factors
-artificial lighting
-heating
-burning fuel to produce CO2
-must be cost effective
4 stages of aerobic respiration
glycolysis (cytoplasm)
link reaction (mitochondrial matrix)
krebs cycle (mitochondrial matrix)
oxidative phosphorylation (cristae)
what steps are in involved in glycolysis
-phosphorylation glucose to glucose phosphate using ATP
-the production of 2x triose phosphate
-oxidation of triose phosphate to produce pyruvate with a net gain of ATP and reduced NAD
-
describe glycolysis
glucose is phosphorylated into hexose bisphosphate using 2x ATP
this then splits into 2x triose phosphate
triose phosphate is oxidised forming 2x pyruvate
2x NAD picks up H to form 2x reduced NAD
4 ATP are prodcuced
what are the products of glycolysis
2x pyruvate
net gain of 2 ATP
2x NADH
describe the link reaction
the pyruvate made in glycolysis is oxidised to acetate
NAD picks up the hydrogen and become reduced NAD
acetate then combines with coenzyme A
what are the products for the link reaction
the link reaction occurs twice for every glucose molecule as 2 pyruvate molecules are made from every glucose molecule in glycolysis
-2 molecules of acetyl coA (goes to Kreb’s cycle)
-2 CO2 molecules (waste product)
-2 molecules of reduced NAD (goes to oxidative phosphorylation)
describe kreb’s cycle
acetyl coA from the link reaction reacts with a 4-C moleucle to form a 6-C molecule
coA goes back to the link reaction to be used again
the 6C compound undergoes a series of enzyme controlled redox reactions involving decarboxylation and dehydrogenation occur where CO2 and hydrogen is removed
the H is used to produce reduced NAD
one molecule of reduced FAD and 2 of reduced NAD are produced
ATP is also produced
what are the product’s per kreb’s cycle
3x reduced NAD
1x reduced FAD
1x ATP
2x CO2
it is double the amount per glucose