Topic 4: Urinalysis Flashcards

1
Q

Can collection procedure of urine effect results of analysis?

A

YES

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2
Q

What are some methods of urine collection?

A

Voiding (free catch)
Cystocentesis
Urethral catheriterisation

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3
Q

What is a disadvantage to voiding?

A

Contamination from the urethra and genitals. Can be avoided by catching midstream.

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4
Q

Samples from cathertirisation may contain what?

A

Blood and epithelial cells

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5
Q

When is ideal time to analyze urine?

A

WIthin 30 mins of collection
If not possible, refrigerate and anaylze within 6 - 12 hours
Bring to room remp and mix before anaylsis

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6
Q

What can affect urine stability?

A

PH, concentration and presence of bacteria

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7
Q

What are the physical and chemical properties of urine?

A

Physical:

  • Colour
  • Transperancy
  • Turbidity
  • USH

Chemical:

  • pH
  • protein
  • glucose
  • ketones
  • bilirubin/urobilirubin
  • occult blood
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8
Q

What is the yellow colour of urine associated with?

A

Renal excretion of plasma urochrome, a yellow soluble sulphur-containing oxidation product of a colourless urochromogen

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9
Q

What are some common causes of increased or abnormal turbidity?

A

Increased cells, numerous crystals, bacteria and lipiduria, presence of mucous, semen and faecel contamination

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10
Q

Colourless urine could indicate?

A

Very dilute - diuretics, diabetes, fluid therapy, overhydration

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11
Q

Milky white urine could indicatE?

A

Lipid
Pyuria
Crystals

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12
Q

Yellow or amber urine could indicate?

A

Urochromes

Urobilin

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13
Q

Deep yellow urine could indicate?

A

Highly concentrated

Lrg quantities of riboflavin

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14
Q

What is urine specific gravity?

A

Ratio of the density of urine to the density of water.

Used to determine whether concentrating ability is adaquete

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15
Q

Urine is specifically concentrated in animals that are what?

A

Conserving water, dehydrated, hypovolaemic or decreased effective blood curculaing volume

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16
Q

What is a normal dog and cat specific gravity?

A

Dog: 1.016 - 1.060
Cat: 1.020 - 1.040

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17
Q

What are reagent strips used for?

A

To determine chemical evaluations like urine, pH, ketones, protein, glucose, bilirubin and blood

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18
Q

Herbivores have alkaline/acidic urine, carnivores have alkaline/acidic urine?

A

Herbivores - alkaline

Carnivores - acidic

19
Q

Why can pH be important?

A

Causes precipitation of some crystals

20
Q

What protein does reagent strip usually detect?

A

Albumin

21
Q

Normal urine should contain how much protein?

A

None to trace

22
Q

WHat are some cases of proteinuria?

A

UTI, haematuria, glomerular disease

23
Q

What are Bence Jones Proteins?

A

Free light chains of immunoglobulins
May be observed in healthy proteins
Increased numbers if there is tubular dysfunction

24
Q

Positive glucose can indicate what?

A

Diabetes
Hyperadrenocoritism
Bacterial cystitis
Stress hypoglycaemia in cats and cattle

25
Q

Ketones are what?

A

By products of fatty acid metabolism

26
Q

Positive ketones can indicate what?

A

Bovine (ketosis)
Pregnancy toxaemia
Diabetes
Starvation or malnutrition

27
Q

What happens when haemoglobin is degraded?

A

Heme portion is converted into bilirubin
Conjugated in liver and excreted in bile
Some conjugated bilirubin is filtered by the glomerulus and excreted in urine

28
Q

When does bilirubinuria occur?

A

When conjugated bilirubin exceeds the renal threshold

29
Q

Positive bilirubin can indicate what?

A

Liver disease

Haemolysis

30
Q

What can heme (blood) on the regaent indicate?

A

Haemorrage
Haemolysis
Skeletal muscle injury

31
Q

How do you prepare urine for microscopic evaluation?

A

Centrifuge
3-5mls
1000-15000rpm for 3-5 mins

Wet Prep: unstained with coverslip
Sedistain: stained with coverslip
Air dried and diff quick

Examination:
100X crystals, cast cells
400X cells bacteria

32
Q

Normal urine should contain how much RBC?

A
33
Q

What type of WBC is usually present in the urine?

A

Neutrophils

34
Q

What can increased WBC indicate?

A

Pyuria due to inflammation, infection, trauma, neoplasia

35
Q

What are the two main varinats of epithelial cells in urine?

A

Squamous (flat cells)

Non- squamous (round central nucelous)

36
Q

What are casts?

A

Elongated, cylindrical structures formed by mucoprotein congealing within renal tubules and may contain cells

37
Q

What type of casts exist?

A
Hyaline (transparent) 
Epithelia cellular casts
Granular casts
Waxy casts 
WBC or RBC casts
38
Q

What does crystal and uroliths presence depend on?

A

Urine pH
Concentration of crystallogenic substances
Temp
Length of time bewteen collection of examination
SPecies and breed
Presence of bacerial cystitis

39
Q

Struvite crysals?

A
Magnesium ammonium phosphate 
Colourless, 3D prisms 
Large and readily observed 
Most common 
Formation favoured in neutral to alkaline urine
40
Q

Calcium carbonate crystals?

A
Variably sized 
Large spheroids with radial striations 
Round to ovoid shapes 
Colourless to yellow brown 
Common in horses, rabbits, goats
41
Q

Calcium oxalate crystals?

A

Dihydrate and monohydrate
DI - Any pH, colourless squares connected by intersecting lines, can be normal and artefact storage

MONO - spindle shapre, variable, urine saturation, urine of healthy horses

42
Q

Cystine crystals?

A

Hexagonal
Flat colourless plates
Aggregate in layers esp in acidic urine
Can be breed specific

43
Q

Uroliths ?

A

Form via a crystal nidus
Named depending on location, components and shape
Most clinical problem related to urethroliths
BLADDER STONES