topic 3 the genome Flashcards

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1
Q

What is DNA?

A

A polymer made up of two strands forming a double helix, strands linked by a series of complementary base pairs joined together by weak hydrogen bonds

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2
Q

what a the monomers of dna?

A

nucleotides

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3
Q

what are DNA nucleotides made up of

A
  • Common sugar
  • Phosphate group
  • One of four bases: A, T, C or G
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4
Q

describe how nucleotides interact to form a molecule of DNA

A

● Sugar and phosphate molecules join to form a sugar-phosphate backbone in each DNA strand
● Base connected to each sugar
● Complementary base pairs (A pairs with T, C pairs with G) joined by weak hydrogen bonds

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5
Q

State the full names of the four bases found in nucleotides

A

Adenine, Thymine, Cytosine, Guanine

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6
Q

define genome

A

The entire genetic material of an organism

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7
Q

What is a chromosome?

A

A long, coiled molecule of DNA that carries genetic information in the form of genes.

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8
Q

define gene

A

segment of DNA that codes for a protein

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9
Q

describe the method used to extract DNA from fruit

A
  1. Place a piece of fruit in a beaker and crush it
  2. Add detergent and salt, mix
  3. Filter the mixture and collect the liquid in a test tube
  4. Pour chilled ethanol into the test tube
  5. DNA precipitates forming a fibrous white solid
  6. Use a glass rod to collect the DNA sample
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10
Q

Why is detergent added to the crushed fruit?

A

It disrupts the cell membranes, releasing DNA into solution

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11
Q

why is salt added to the crushed fruit?

A

salt encourages the precipitation of DNA

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12
Q

Why is chilled ethanol added rather water?

A

DNA is insoluble in ethanol, encouraging its precipitation

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