topic 3 the genome Flashcards
What is DNA?
A polymer made up of two strands forming a double helix, strands linked by a series of complementary base pairs joined together by weak hydrogen bonds
what a the monomers of dna?
nucleotides
what are DNA nucleotides made up of
- Common sugar
- Phosphate group
- One of four bases: A, T, C or G
describe how nucleotides interact to form a molecule of DNA
● Sugar and phosphate molecules join to form a sugar-phosphate backbone in each DNA strand
● Base connected to each sugar
● Complementary base pairs (A pairs with T, C pairs with G) joined by weak hydrogen bonds
State the full names of the four bases found in nucleotides
Adenine, Thymine, Cytosine, Guanine
define genome
The entire genetic material of an organism
What is a chromosome?
A long, coiled molecule of DNA that carries genetic information in the form of genes.
define gene
segment of DNA that codes for a protein
describe the method used to extract DNA from fruit
- Place a piece of fruit in a beaker and crush it
- Add detergent and salt, mix
- Filter the mixture and collect the liquid in a test tube
- Pour chilled ethanol into the test tube
- DNA precipitates forming a fibrous white solid
- Use a glass rod to collect the DNA sample
Why is detergent added to the crushed fruit?
It disrupts the cell membranes, releasing DNA into solution
why is salt added to the crushed fruit?
salt encourages the precipitation of DNA
Why is chilled ethanol added rather water?
DNA is insoluble in ethanol, encouraging its precipitation