Topic 3 - Lesson 3+4

Question 1

Magnification

Answer 1

How many times the image has been enlarged

Question 2

Resolution

Answer 2

A microscopes ability to distinguish between two points that are close together(the fine detail).

Question 3

Light Microscope

Answer 3

It uses light to form an image, magnified and focused with objective and eyepiece lenses
Maximum Magnification - x1500
Resolution - 0.2 Micrometres
Advantages - Affordable, Portable, can look at a living species
Disadvantages - Lower magnification and resolution than electron microscope

Question 4

Electron Microscope

Answer 4

Use a beam of electrons to form an image
Maximum Magnification - x1500000
Resolution - 0.0002 Micrometres
Advantages - Higher magnification and resolution than light microscope
Disadvantages - Cannot look at living species

Question 5

TEM Microscope

Answer 5

Beam of electrons focused using electromagnets
Maximum Magnification - x1500000
Resolution - 0.0002 Micrometres
Advantages - Higher resolution than SEM and can see variation in sample density
Disadvantages - Samples must be very thin images only 2D

Question 6

SEM Microscope

Answer 6

SEMs scan a beam electrons across a specimen. This knocks off electrons from the specimen, which are gathered in a cathode ray tube to form an image.
Maximum Magnification - x1500000
Resolution - 0.0002 Micrometres
Advantages - Can look at thicker images and produces 3D images
Disadvantages - Lower resolution than TEM

Question 7

Labelling a Microscope

Answer 7

https://www.pinterest.co.uk/pin/5699937006745863/

Question 8

+Protein Transport within cells

Answer 8

1. Proteins are made in the ribosomes. New proteins produced by the ribosomes on the rough endoplasmic reticulum are folded and processed in the rough ER.
2. Proteins are then transported to the Golgi apparatus after being packaged into vesicles.
3. At the Golgi, the proteins undergo further modification. The proteins enter more vesicles to be transported out of or around the cell.
4. Extracellular enzymes are secreted as the secretory vesicle fuses with the membrane during exocytosis.