Topic 2: Genetic Code and Mutation Flashcards
____: adenine and guanine
purines
____: cytosine, thymine, uracil
pyrimidines
each strand of DNA is composed of ____ and _____
nucleotides and sugar-phosphate backbone
where does the phosphodiester bond occur in DNA? between what two ends?
the 5’ phosphate group of a nucleotide and the 3’ hydroxyl group of the adjacent nucleotide
bases are _______
hydrophobic
the complementary strand runs _____ to the main strand
anti-parallel
__________: the theory that each new DNA duplex has one parental strand and one daughter strand
semi-conservative replication
purine nucleotides are _____-ringed
double
pyrimidine nucleotides are _____-ringed
single
how many base pairs twist with each turn of the double-helix?
10 base pairs/turn
the proportion of each base pairing is ______
equal (30% A = 30% T)
DNA replication is _____, forming a replication bubble
bidirectional
prokaryotes have a ______ origin of replication
single, because they have a circular genome!
eukaryotes have _______ origins of replication
multiple, because they have linear DNA
in what order do these proteins work in DNA replication?
- DNA polymerase III
-DNA polymerase I
- helicase
- primase
- SSB
- DNA topoisomerase
- DNA ligase
- DNA topoisomerase
- Helicase
- SSB
- Primase
- DNA poly III
- DNA poly I
- DNA ligase
what does DNA topoisomerase do?
relaxes supercoiling
what does helicase do?
unwinds the double helix
what does SSB do?
prevents re-annealing of separated strands
what does primase do?
synthesizes RNA primers
what does DNA poly III do?
synthesizes DNA
what does DNA poly I do?
removes and replaces RNA primer with DNA (cuts out nucleotides)
what does DNA ligase do?
joins DNA segments together
what direction is the template strand read in DNA replication?
3’ - 5’, new strand elongates in the 5’ -3’ direction!
most of the proteins/enzymes required for replication are apart of the complex called ______
replisome
replication is done in chunks… what are these “chunks” called?
okazaki fragments!
DNA polymerase I uses a 5’->3’ ______ activity to remove RNA nucleotides
exonuclease
DNA polymerase uses a 5’->3’ ______ activity to add DNA nucleotides
polymerase
true/false: DNA poly I and DNA ligase are active on both the leading and lagging strand
true! they’re just more prominent on the lagging strand
true/false: telomeres in prokaryotes
false! they’re only in eukaryotes
germ-line cells contain ____ that adds repetitive DNA sequences to the ends of telomeres
telomerases (enzyme containing RNA)
what is the Hayflick limit?
the number of repetition cycles in a cell’s life span (~50-70 cycles)
each successful replication ______ the telomeres in somatic cells
shortens
what is the central dogma of genetics?
the flow of heredity information (DNA -> transcription -> RNA -> translation -> protein)
______: DNA is converted to mRNA
transcription
in transcription, the new RNA molecule is synthesized via _________
RNA polymerase
what are the three stages of transcription?
- initiation
- elongation
- termination
what is the complementary DNA strand to the template strand called?
coding strand