topic 2 - cells and viruses Flashcards
what does cell theory state?
cells are a fundamental unit of structure, function and organisation in all living organisms
what magnification can a light microscope do?
x1500
what magnification can an electron microscope do?
x50,000
define magnification
how many times bigger than image is than the real thing
define resolution
measure of how close two objects can be before we see them as one
what does haematoxylin do? (stain)
stains nuclei purple/blue/brown
what does methylene do? (stain)
stains nuclei blue
what does acetocarmine do? (stain)
stains chromosomes in dividing nuclei
what does iodine do? (stain)
stains starch containing material (in plants) blue/black
what are the advantages of a light microscope?
can see living specimens
cheap
portable
what are the disadvantages of light microscopes?
preservation can cause artefacts
limited resolution and magnification
name a stain that can be used to show the different stages of mitosis
orcein
advantages of electron micrographs
high magnification and resolution
see very detailed image
disadvantages of electron micrographs
specimens are dead (vacuum)
treatments- artefacts and very skilled work
expensive
large- need constant temp and pressure
how can you make a specimen more visible?
warm to intensify stain
tease cells apart
add stain
what does the cell surface membrane do?
controls what enters/leaves the cell
why do some membranes need to be able to break/fuse easily?
to allow vesicles (carrying chemical secretions) out of the cell
what are membranes mainly made from?
lipids and proteins
what are polar lipids?
lipid molecules with one end joined to a polar group
how does the proportion of phospholipids unsaturated containing fatty acids affect the fluidity of the membrane?
the more unsaturated fatty acids, the more fluid it is because they are kinky so are liquid at room temperature
what are gated channels?
channels that can open/shut depending on the conditions of the cell
what is the protoplasm?
combination of cytoplasm and nucleus
give examples of what cell membranes do
controls what enters/leaves
localises enzyme pathways
what is the affect of the nucleus having pores?
allows chemicals to pass in/out so nucleus can control reactions
what substances are inside the nuclear envelope?
nucleic acids and proteins
what are the nucleic acids?
DNA and RNA
when the cell is not actively dividing, what does DNA bind to?
chromatin
what is the nucleolus and what is it involved in?
the nucleolus is an area in the nucleus containing extra dense DNA and protein
it is involved in the production of ribosomes
how many membranes does a mitochondria have?
two- an out and inner membrane
inner membrane- folded- larger SA
surrounded by fluid matrix
what is special about mitochondria and DNA?
mitochondria contain their own genetic material so when a cell divides, mitochondria replicate themselves
what is a centriole made from?
9 tubules
what is the role of the centrioles?
involved in cell division
pull apart to create spindle of microtubules- these are involved in the movement of chromosomes
what is the cytoskeleton?
3D web like structure
contains microfilaments and microtubules
what is the function of the cytoskeleton?
gives cytoplasm structure
keeps organelles in place
cell movement and transport
what are contractile vacuoles?
vacuoles that empty/fill to help maintain concentration of cytoplasm (in freshwater animals)
where are thylakoids found?
chloroplasts
what is translocation?
when part of a chromosome breaks off and attaches to another chromosome
what is the type of chromosome mutation that results in Down’s syndrome?
non-disjunction
what ribosomes do eukaryotic cells contain?
80s ribosomes
what are the two subunits for 80s ribosomes?
60s and 40s
what are the two subunits for 70s ribosomes?
30s and 50s
what is the function of the RER?
isolates and transports proteins (once they’ve been made by the ribosomes)
what is the role of the SER?
synthesis and transport of lipids and steroids
what is the golgi apparatus made from?
lots of flatterned stacks of cisternae
(formed by vesicles from ER fusing together)
what does the golgi apparatus do?
proteins brought there (that have pinched off ER)
vesicles fuse with membrane sacs of golgi apparatus and proteins enter
as they travel through, the proteins are modified
what is the role of lysosomes?
contain enzymes that break down molecules
what is apoptosis?
controlled cell death- lysosomes release their contents
what is the first layer to form when a plant cell divides?
middle lamella
explain how calcium pectate is formed
pectin has n….. charged car….. groups
these combine with positive calcium ions to form ……
this binds to the cellulose that forms on either side
pectin has negatively charged carboxyl groups
these combine with positive calcium ions to form calcium pectate
this binds to the cellulose that forms on either side
what is the difference between the primary and secondary cell wall?
primary: more flexible, microtubules are all laying in same direction
secondary: more built up, more rigid, microtubules are laying at different angles- hemicelluloses harden it further
what is added to cell walls to produce wood?
lignin
what are plant fibres? uses?
long cells in cell walls that have been heavily lignified - used in clothing/ropes/paper
what are plasmodesmata?
cytoplasm bridges which allow for communication between cells
what is the symplast?
the interconnected cytoplasm of the cells
what happens in exocytosis?
vesicle fuses with cell surface membrane and substances exits the cell
what is a vacuole?
a fluid filled space inside the vacuole with a cell membrane
how frequently do vacuoles form in animal cells?
vacuoles form frequently in animal cells but they are only temporary and therefore only for, when they are needed
what is a vacuole’s membrane called?
tonoplast