Topic 1B- More Biological Molecules Flashcards
What is the function of DNA?
Used to store genetic information
What is the function of RNA?
To transfer genetic information from the DNA to the ribosomes
DNA and RNA are polymers of what type of biological molecule?
Nucleotides
What are nucleotides made up of?
- a pentose sugar
- a nitrogen-containing organic base
- a phosphate group
What are the four bases in DNA?
Adenine
Thymine
Cytosine
Guanine
Name 4 differences between DNA and RNA
- RNA contains nucleotides with a ribose sugar not deoxyribose
- RNA is single stranded, DNA is double stranded
- RNA has the base uracil while DNA has the base thymine
- RNA is much shorter than DNA polynucleotides
What type of reaction do nucleotides join via?
Condensation, between the phosphate group of one nucleotide and the sugar of another
What bond is formed between a phosphate of one nucleotide and the sugar of another?
Phosphodiester bond
What is the sugar-phosphate backbone?
A chain of sugars and phosphates
What are the base pairs in DNA?
AT
CG
What type of bond is formed between bases?
Hydrogen
DNA strands are antiparallel. What does this mean?
They run in opposite directions.
What type of replication does DNA replicate by?
Semi-conservative replication
Describe what happens in DNA replication
- Enzyme DNA helicase breaks down hydrogen bonds between bases on the two polynucleotide strands
- Each original single strand acts as a template for a new strand. CBP means that free floating DNA nucleotides are attracted to their complementary bases on the original template strand
- Condensation reactions join the nucleotides of the new strands together- catalysed by the enzyme DNA polymerase. Hydrogen bonds form between bases between new and original strands.
Why does DNA polymerase move in opposite ways along DNA strands?
Each end of DNA is called 3’ and 5’
The active site of DNA polymerase is only complementary to the 3’ end. So the enzyme can only add nucleotides to the new strand at the 3’ end.
Meelson and Stahl used to isotopes of what element to prove DNA replication was semi-conservative?
Nitrogen- 15N and 14N
Describe Meelson and Stahl’s experiment
1) Two samples of bacteria were grown in a nutrient broth containing light nitrogen and heavy nitrogen. As bacteria reproduced, they took up nitrogen from the broth to help make nucleotides for the new DNA.
2) A sample of DNA was taken from each batch of bacteria, and spun in a centrifuge. The DNA from the heavy nitrogen bacteria settled lower down the centrifuge tube because its heavier.
3) Then the bacteria grown in the heavy nitrogen broth were taken out and put in the broth containing only light nitrogen. They were left for one round of replication, and then taken out and spun in the centrifuge.
4) DNA settled out in the middle, showing it contained a mixture of light and heavy nitrogen.
What is ATP?
The immediate source of energy in a cell