Topic 16 - Amino Acid Metabolism Flashcards

1
Q

All amino acids contain ….. which exists in 3 forms. These forms are ……., ……… and ……….

These forms are interconverted in the ….. cycle

A

All amino acids contain nitrogen, which exists in 3 forms.

These forms are unfixed (gaseous), inorganic (NH4+) and organic (aa).

These forms are interconverted in the nitrogen cycle.

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2
Q

Describe the nitrogen cycle

A
  • Unfixed N2 => fixed NH4+ by n-fixing bacteria & algae
  • Fixed N NH4+ used by plants to form aa/proteins
  • aa consumed by animals
  • animals excrete excess N into enviro as ammonia in urea
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3
Q

Give a brief overview of aa metabolism

A
  • aa metabolism involves seperating amino group from carbon group, which is achieved by amination, deamination & transamination reactions
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4
Q

Describe Amination

A
  • Glutamine synthetase catalyses addition of ammonium onto glutamate to form glutamine
  • Results in temporary amination to allow transport of ammonium in blood as glutamine (NH4+ is toxic)
  • In liver, NH4+ is re-released by glutaminase, & disposed of in urea
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5
Q

Describe Deamination

A
  • Reverse of amination
  • Two important enzymes:
  • 1. Glutaminase (liver, kidney cortex)*
  • Removal of ammonia from glutamine reforming glutamate (ammonium => urea in liver)
  • Ammonia produced derived from amide N of glutamine (side chain)
  • 2. Glutamate DH (liver)*
  • Removal of amino group from glutamate to form a-keto glutarate & ammonium
  • Rxn is reversible, equilibrium constant favours deamination rxn
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6
Q

Describe Transamination

A
  • aa transfers its amino group to a-keto glutarate & is converted to an a-keto acid
  • Glutamate formed
  • No net deamination (loss of amino group) -a-keto g is aminated; aa deaminated
  • For most aa there is a specific aminotransferase that transfers amino group onto a-keto g
  • Rxn readily reversible
  • All amintransferases need pyridoxal phosphate (PLP) as a cofactor
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7
Q

Describe amino acid catabolsim -in 4 easy steps!
When is it a major energy source?

A
  • Is NOT a major energy source under NORMAL conditions
  • Significant under 3 conditions:
  • **-dietary protein ingested in excess
  • severe starvation
  • stress situations***
  • Catabolism in 4 easy steps!
  • 1.* catabolism of protein w/in muscle results in formation of aa’s **glutamine & alanine
    2. **
    Transport of Glu & Ala into blood
  • Ala => liver
  • Glu => liver & kidney
  • 3. Kidney -* Glu deaminated twice => a-keto g. Ammonium excreted in urine. C skeleton used in glucose formation
  • 4. Liver -* Ala & Glu from muscle, & all 20 aa from diet are deaminated => glutamate. C skeleton => glucose or ketone body. Glutamate deamin. in mitochondria & NH4+ converted to urea. Urea => liver => blood => kidney => excretion
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8
Q

DRAW THE DIAGRAM THAT SUMMARISES AA CATABOLISM

Remember to include:
Where there us organic or inorganic N
transamination rxns
deamination rxns
PLP
Where NH4+ is produced/released
muscle/kidney/liver cell section
mitochondria cell section

A

Don’t forget extra labels written in lecture

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9
Q

Brief overview of C skeleton degradation?

A

Glucogenic aa - c skeleton fed into GNG pathway

Ketogenic aa - c skeleton used to make ketone bodies

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10
Q

aa syntesis -essential and non-essential aa’s?

A
  • Humans and animals can catabolise all 20 aa, but not all possess the pathways to synthesise all 20 aa’s
  • Animals typically cannot synthesise 8-12 of the aa & must obtain them from diet. These are called essential amino acids
  • Humans can only synthesise 10 aa. These are called non-essential amino acids as they are not essential in the diet
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11
Q
A
  1. PLP
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12
Q
A
  1. liver
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13
Q
A
  1. Very low CHD, very high protein
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14
Q
A
  1. Glutamine and alanine
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