topic 1 - enzyme controlled reactions

Question 1

what is the first step to measuring the rate of an enzyme controlled reaction

Answer 1

set up boiling tubes containing the same volume and concentration of hydrogen peroxide - to keep pH constant add equal volumes of a suitable buffer solution to each tube

Question 2

what is the second step to measuring the rate of an enzyme controlled reaction

Answer 2

set up the rest of the apparatus - a trough of water with an upside down measuring cylinder inside - a boiling tube containing hydrogen peroxide solution and catalase enzyme with a bung at the opening and a delivery tube attached to that, which has been also put through the upside down measuring cylinder

Question 3

what is the third step to measuring the rate of an enzyme controlled reaction

Answer 3

put each boiling tube in a water bath set to a different temperature along with another tube containing catalase - wait 5 minutes before moving onto the next step so the enzyme get up to temperature

Question 4

what is the fourth step to measuring the rate of an enzyme controlled reaction

Answer 4

use a pipette to add the same volume and concentration of catalase to each boiling tube - then quickly attach the bung and delivery tube

Question 5

what is the fifth step to measuring the rate of an enzyme controlled reaction

Answer 5

record how much oxygen is produces in the first minute of the reaction - use a stopwatch to measure the time

Question 6

what is the sixth step to measuring the rate of an enzyme controlled reaction

Answer 6

repeat the experiment at each temperature three times and use the results to find an average volume of oxygen produced

Question 7

what is the seventh step to measuring the rate of an enzyme controlled reaction

Answer 7

calculate the average rate of reaction at each temperature by dividing the volumes produced by the time taken