Tom Richards

Question 1

if the paper is comparing two things

Answer 1

maybe it is looking for homology?

Question 2

think about the organism being used

Answer 2

• is it easy to transform
• can you culture it?

Question 3

instead of saying knock in, say

Answer 3

heterologous complementation assay

Question 3

isolating things to measure them = bad. for mitochondria, you could use

Answer 3

Mitoferrofluor (MFF) or Mito-FerroGreen to detect and quantify chelatable iron (Fe2+)

Question 4

antibody localisation

Answer 4

• primary and secondary antibodies don’t cross react with other proteins
• this has to be checked using western blotting
• supplement with quantitative image analysis

Question 5

laser scanning confocal microscopy is highly advanced due to its

Answer 5

high spatial resolution and signal-to-noise ratio

Question 6

what has higher resolution that laser confocal microscopy>

Answer 6

immuno-gold electron microscopy

Question 7

you can’t conduct a phylogeny on things that

Answer 7

don’t have a genome

Question 8

if you use the terms “necessary” and “sufficient”

Answer 8

explain what they mean - you can’t assume that the marker will give you the benefit of the doubt