To prepare an enzyme immobilisation and examine its application

Question 1

Briefly explain the term enzyme.

Answer 1

biological catalyst

Question 2

3 advantages of immobilising enzymes?

Answer 2

pure product
easily recovered
reusable

Question 3

Name the enzyme used and how it was sourced.

Answer 3

enzyme: sucrase
source: yeast

Question 4

During preparation, firstly mix yeast with what in what? When do you stop mixing?

Answer 4

with water in a beaker until smooth

Question 5

After mixing yeast and water what do you add to a second beaker until smooth?

Answer 5

sodium alginate dissolved in water

Question 6

After you have your beakers mixed what do you do?

Answer 6

mix the two beakers together

Question 7

After mixing the two beakers together, using a ___, what do you do?

Answer 7

with a syringe, draw solution up and drop into a beaker containing calcium chloride and water (calcium chloride)

Question 8

With a syringe, add dropped solution of mixed beakers to what?

Answer 8

into a solution of calcium chloride

Question 9

Once the mixture was dropped into a solution of calcium chlroide what happens?

Answer 9

beads harden

Question 10

What is an immobilised enzyme?

Answer 10

enzyme that is attached/fixed to each other
/
an enzyme in a gel

Question 11

Once the beads have hardened what do you do?

Answer 11

rinse the beads