To investigate the effect of temperature on the rate of enzyme activity

Question 1

What do you do first with celery?

Answer 1

weigh it on a mass balance

Question 2

After weighing the celery what do you do?

Answer 2

blend it with water

Question 3

After blending the celery and water, what do you do?

Answer 3

filter celery with coffee filter paper, funnel and a beaker

Question 4

Where do you place the celery filtrate?

Answer 4

a graduated cylinder

Question 5

After adding the celery filtrate to a graduated cylinder, what do you add?

Answer 5

a few drops of washing up liquid

Question 6

After adding washing up liquid, what do you add to the celery filtrate?

Answer 6

pH buffer 9

Question 7

After adding pH buffer and washing up liquid to graduated cylinder what do you do?

Answer 7

place it into an ice bath @0 degrees C

Question 8

What do you put into a second graduated cylinder? Where do you put this second graduated cylinder?

Answer 8

hydrogen peroxide, then into ice bath

Question 9

Once both graduated cylinder reach 0 degrees C, what do you do?

Answer 9

add hydrogen peroxide to celery filtrate and start timer

Question 10

Record the volume of what every minute for 3 minutes?

Answer 10

production of foam volume

Question 11

Repeat the experiment but change what?

Answer 11

using different temperatures (water baths @ 25, 40, 60

Question 12

2 takeaways/results were?

Answer 12

most foam @25 degrees C = optimum temperature

no foam @0, 40, 60 degrees C as catalase enzyme was denatured

Question 13

What enzyme did you use? How did you source this?

Answer 13

catalase, celery