Tissue Prep Flashcards by Nate kalinowski

Q

SEM (visibility)

A

gold-carbon film coating

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Q

TEM (fixative, embedding)

A

Glutaraldehyde/ osmium tetroxide, methacrylate polymer

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Q

Proteins (fixative)

A

formalin

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Q

Collagen & actin (microscope)

A

polarizing

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Q

Phosphatases (method)

A

lead ions/ enzymatic histochemistry

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Q

Special stains (embedding)

A

methylmethacrylate

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Q

Glycogen (stain)

A

PAS

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Q

Carbohydrates (staining)

A

PAS

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Q

Cytoplasmic RNA (stain)

A

hematoxylin

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Q

Basement membrane (stain)

A

PAS

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Q

Reticular fibers (2 staining methods)

A

PAS, silver treatment

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Q

Phospholipids (fixative)

A

osmium tetroxide

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Q

Extracellular fibers (stain)

A

Eosin

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Q

Mucus (stain)

A

PAS

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Q

Stoichiometric (stain)

A

Feulgen reaction

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Q

TEM (fixative, stain)

A

osmium tetroxide, uranyl acetate

Q

Nerve fibers (stain)

A

silver stain

Q

Goblet cells (stain)

A

PAS

Q

Elastic fibers (stain)

A

Orcein

Q

Allows living tissue

A

Phase contrast, AFM

Q

3D images

A

Confocal, SEM, AFM