Tissue Culture & Immunocytochemistry Part 2/2

Question 1

Immunocytochemistry refers to…

Answer 1

Cultured cells that are being stained

Question 2

Immunohistochemistry refers to…

Answer 2

Tissue sections

Question 3

What 2 ways can we view sub-cellular structures?

Answer 3

Histological dyes and antibodies

Question 4

What is confocal fluorescent microscopy?

Answer 4

Allows single optical section to be seen (out of focus light reduces definition) [computer processing required]

Question 5

What is confocal fluorescent microscopy mainly used to image

Answer 5

Thick tissue samples (components within cells are relatively flat- not ideal)

Question 6

2 ways if classifying antibodies by structure?

Answer 6

Light chain + heavy chain

Variable region and Fc region/constant region

Question 7

What are:

variable region:
Fc region/constant region:

Answer 7

variable region: binds to target material (immunogen/antigen)]- different in each antibody

Fc region/constant region: same for all antibodies of a species