Tissue Culture Flashcards
What is in-vitro growth?
The growth of cells (or tissues) outside the body in an artificial environment is called in vitro growth
The growth of cells (or tissues) outside the body in an artificial environment is called what?
In vitro growth
What is the basis on which tissue culture generally works?
Tissue culture generally works on the basis that a sample of a tissue is removed from a plant or animal.
The tissue sample is grown in glassware, or in a bath or bioreactor, under carefully controlled conditions
Where are cells generally grown outside of the body?
Cells are grown in a bath of sterile fluid containing a source of nutrition
Why must the environment for the cells to grow be sterile? Why is this necessary?
This is necessary as micro-organisms might grow faster than the desired cells and they might contaminate the product.
Why are nutrients needed for cells to grow?
To allow the cells to gow
What is commonly added to enhance growth in tissue culture?
It is common to also include hormones and other substances to enhance growth.
Define tissue culture
Tissue culture is the growth of cells in or on a sterile nutrient medium outside an organism
