THREE GREEN Flashcards
Which of the following is an elicited effect of the process dehydration?
a. Harden tissue
b. Remove fixative and water
c. Stabilization of proteins
d. Make tissue clear
b. Remove fixative and water
Embryonic and animal tissues should start the dehydration process in the initial concentration of ___?
a. 65%
b. 30%
c. 70%
d. 95%
b. 30%
Best general decalcifying agent.
a. Phloroglucin nitric acid
b. Von Ebner’s
c. 5% Formic acid
d. Flemming’s fluid
c. 5% Formic acid
Which of the following fixative is used to
preserve sputum?
a. Methyl alcohol
b. Alcoholic Formalin
c. Isopropyl alcohol
d. Ethyl alcohol
b. Alcoholic Formalin
Most rapid decalcifying agent.
a. Von Ebner’s
b. Perenyi’s fluid
c. Formol nitic acid
d. Phloroglucin nitric acid
d. Phloroglucin nitric acid
How many changes of paraffin wax is
essential to remove the clearing agent in an automatic tissue processor?
a. 3-5
b. 1-3
c. 1-2
d. 2-3
d. 2-3
Which of the following is a known function of Perenyi’s fluid?
a. Either
b. Neither
c. Fixative
d. Tissue softener
d. Tissue softener
Which of the following technique is carried out on the knife to remove burr?
a. Stropping
b. Polishing
c. Trimming
d. Honing
a. Stropping
Which of the following decalcifying agents is not suitable for fluids containing mineral acids?
a. Chelating agents
b. Electrophoresis
c. Ion exchange resins
d. Acid decalcifying agents
c. Ion exchange resins
Plane concave knife can be used to cut tissue sections embedded using ____.
a. Paraffin
b. Neither
c. Celloidin
d. Either
d. Either
Which of the following person first
introduced the use of paraffin in wax
embedding procedure?
a. Pappenheimer
b. Papanicolaou
c. Butschlii
d. Walter
c. Butschlii
The eosin has high affinity for ____
components in the cell.
a. Basic
b. Neutral
c. None of these
d. Acidic
a. Basic
Which of the following technique is carried out on the knife to remove gross nicks?
a. Polishing
b. Trimming
c. Honing
d. Stropping
c. Honing
Which among the choices is the correct way of carrying out the honing procedure?
a. 30 double strokes toe to head
b. 30 double strokes heel to toe
c. 30 double strokes head to toe
d. 30 double strokes toe to heel
b. 30 double strokes heel to toe
Which among the choices is the correct way of carrying out the stropping procedure?
a. 30 double stroke toe to heel
b. 40 double stroke heel to toe
c. 30 double stroke heel to toe
d. 40 double stroke toe to heel
d. 40 double stroke toe to heel
Best known aldehyde fixative.
a. Formol corrosive
b. 10% Neutral buffered formalin
c. Karnovsky’s paraformaldehydeglutaraldehyde
d. Acrolein
c. Karnovsky’s paraformaldehydeglutaraldehyde
Which of the following step is implored to get rid of the unwanted artifacts upon staining?
a. Dealcoholization
b. Differentiator
c. Deparaffinization
d. Washing out
d. Washing out
Which of the following techniques can hasten decalcification process?
a. All of these
b. Greater amount of decalcifying agent
c. Application of heat
d. More concentrated decalcifying agent
a. All of these
Decalcification process using ion exchange resins can be hasten using ___?
a. Tricholoroacetic acid
b. Formic acid
c. Formol nitric acid
d. Chromic acid
b. Formic acid
Denatured alcohol is a compound of what alcohol/s?
a. Either
b. Isopropyl alcohol
c. Methyl alcohol
d. Neither
c. Methyl alcohol
Which of the following decalcifying agents is ideal for immunohistochemical staining in tissue sections?
a. Ion exchange resins
b. Acid decalcifying agents
c. Electrophoresis
d. Chelating agents
d. Chelating agents
Which of the following embedding medium can be used to the tissue even without prior dehydration and clearing of the tissue specimen?
a. Paraplast
b. Carbowax
c. Paraffin
d. Esterwax
b. Carbowax
A medical technologist happens to proceed with decalcification following a temperature of 37°C. Which of the tissue elements would not be visible if Van Gieson’s stain was implored?
a. None of these
b. Fibroelastic fibers
c. Collagen fibers
d. Elastic fibers
c. Collagen fibers
Which of the following embedding medium that would be used will take take long period of time to be accomplished?
a. Paraplast
b. Cellloidin
c. Carbowax
d. Tissue mat
b. Cellloidin
Which of the following chemical is added to gelatin to inhibit the growth of molds?
a. Phenol
b. Ethanol
c. Methanol
d. Resin
a. Phenol
A part of the automatic tissue processor
responsible for moving the tissue specimen from a processing reagent to another.
a. Probe
b. Beam balance
c. Transfer foot
d. Transfer arm
d. Transfer arm
Ideally to facilitate a quality embedding
produce paraffin should be melted in a
paraffin oven with a temperature ________ the melting point of the paraffin wax used.
a. 4-6°C below
b. 2-5°C above
c. 2-5°C below
d. 4-6°C above
b. 2-5°C above
Which of the following fixatives can fix bone marrow biopsies for 1.5 to 2 hours?
a. Zenker’s formol
b. Schaudinn’s fixative
c. B5 fixative
d. Heidenhain Susa
c. B5 fixative
Which of the following techniques can cause damage to the tissue being processed for decalcification?
a. All of these
b. More concentrated decalcifying agent
c. Greater amount of decalcifying agent
d. Application of heat
a. All of these
Completeness of the decalcification process is measured through poking a rod or a stick unto the tissue to approximate.
a. Xray method
b. None of these
c. Physical method
d. Chemical method
c. Physical method
Which of the following is a part of an
automatic tissue processor responsible for controlling the time necessary for each processing step?
a. Mechanical clock
b. Digital clock
c. Electrical clock
d. Alarm clock
c. Electrical clock
Which of the following embedding medium is recommended for whole eye section?
a. Carbowax
b. Paraplast
c. Ester wax
d. Bioloid
d. Bioloid
Which of the following is an ideal pH for
fixation?
a. 5-9
b. 5-7
c. 6-8
d. 6-9
c. 6-8
Which of the following honing stone is used for badly nicked and jagged knives?
a. Belgium yellow
b. Fine carborandum
c. Whetstone
d. Arkansas
b. Fine carborandum
Which of the following embedding medium can be used to the tissue even without prior clearing of the tissue specimen?
a. Ester wax
b. Paraplast
c. Embeddol
d. Tissue mat
a. Ester wax
Which of the following dehydrating agents is used in the routine process of dehydrating tissue samples?
a. Methyl alcohol
b. Isopropyl alcohol
c. Ethyl alcohol
d. Butyl alcohol
c. Ethyl alcohol
Tissue in fixation is preserved by preventing the following except.
a. Hardening
b. Putrefaction
c. Distortion
d. Degeneration
a. Hardening
A medical technologist submerges a tissue specimen at a decalcifying agent following a temperature of 55°C. What would more likely happen to the tissue after 24 hours.
a. Tissue will become soft
b. Tissue will have impaired nuclear
staining
c. Tissue will have impaired cytoplasmic
staining
d. Tissue will undergo complete
digestion
d. Tissue will undergo complete
digestion
Fixes connective tissue mucin and is
recommended for acid mucopolysaccharides.
a. Chromate fixatives
b. Mercuric chloride fixatives
c. Picric acid fixatives
d. Lead fixatives
d. Lead fixatives
What is the maximum amount of frequency that a paraffin wax can be reused no matter if it was filtered?
a. 1x
b. 3x
c. 5x
d. 2x
d. 2x
Which of the following is not an ideal fixative characteristic?
b. Harden tissue
c. Produce minimum shrinkage
d. Inhibit bacterial decomposition
a. Provide distortion
Which of the following technique is carried out on the knife to remove gross irregularities in the tissue section during microtomy?
b. Polishing
c. Trimming
d. Stropping
a. Honing
Considered as the simplest among the types of microtomes.
a. Rotary microtome
b. Freezing microtome
c. Sliding microtome
d. Rocking microtome
d. Rocking microtome
Which of the following decalcifying agents is ideal for enzyme staining in tissue sections?
a. Electrophoresis
b. Acid decalcifying agents
c. Ion exchange resins
d. Chelating agents
d. Chelating agents
Which of the following agent is known to be toxic to the liver upon prolonged exposure?
a. Chloroform
b. Benzene
c. Xylene
d. Toluene
a. Chloroform
Which of the following clearing agent is
known to cause damage on the bone marrow upon prolonged exposure and may potential cause aplastic type of anemia.
a. Chloroform
b. Benzene
c. Xylene
d. Toluene
b. Benzene
Which of the following is a source for the
stain hematoxylin?
a. Blood
b. Insect
c. Moss
d. Tree
d. Tree
Which of the following among the choices is not a freezing agent?
b. Carbon dioxide
c. Liquid nitrogen
d. Aerosol spray
a. Isohexane
Sections to be cut using the ultrathin
microtome are fixed using ___.
a. Gluteraldehyde
b. Formol saline
c. 10% Formalin
d. Osmium tetroxide
d. Osmium tetroxide
Which of the following fixative is best used for iron containing granules tissue?
a. Formol calcium
b. 10% Neutral buffered formalin
c. 10% Formol saline
d. Formol corrosive
b. 10% Neutral buffered formalin
Which of the following aldehyde fixatives
contains mercuric chloride?
a. Glutaraldehyde
b. Acrolein
c. Formol corrosive
d. Formol calcium
c. Formol corrosive
A biconcave knife is ideally used using a ___.
a. Sliding microtome
b. Rocking microtome
c. Freezing microtome
d. Rotary microtome
d. Rotary microtome
Which of the following is the major
disadvantage of using the rocking microtome?
a. Sections are liable to disintegrate
b. Turning the ratchet feed wheel
c. Reorienting the block
d. Inclining the pawl
c. Reorienting the block
The technician is currently on the embedding stage of the tissue processing to facilitate producing a quality tissue block the technician must ensure that the paraffin oven
temperature must be kept at ______.
a. 60-65°C
b. 45-50°C
c. 55-60°
d. 50-55°C
c. 55-60°
Which among the honing stone gives a better polishing effect?
a. Fine carborandum
b. Belgium yellow
c. Whetstone
d. Arkansas
Excellent fixative for glycogen
a. Ohlmacher’s
b. Brasil’s alcoholic picroformol fixative
c. Orth’s fluid
d. Chromic acid
b. Brasil’s alcoholic picroformol fixative
Rehydration of the tissue section is done
using _____.
a. Descending grade of xylene
b. Ascending grade of xylene
c. Ascending grade of alcohol
d. Descending grade of alcohol
d. Descending grade of alcohol
Which of the following classes of alcohol can be used in a dehydration process imploring the microwave technique?
a. Isopropyl alcohol
b. Butyl alcohol
c. Ethyl alcohol
d. Denatured alcohol
a. Isopropyl alcohol
The hematoxylin has high affinity for ____components in the cell.
a. Basic
b. None of these
c. Acidic
d. Neutral
c. Acidic
Which of the following is a correct tissue to fluid ratio in the process of decalcification?
a. 1:20
b. 10:1
c. 1:10
d. 20:1
a. 1:20
What would be the appropriate melting point of the paraffin if the current laboratory temperature is at 16°C.
a. 54°C
b. 55°C
c. 57°C
d. 56°C
a. 54°C
Which of the following mechanism hasten the impregnation process by removing air bubbles within the tissue such as lungs?
a. Infrared
b. Heat
c. Vacuum
d. Ultraviolet
c. Vacuum
Which of the following process would
describe a tissue being arranged in precise positions in the mold?
a. Orientation
b. Embedding
c. Blocking
d. Casting
a. Orientation
Which of the following clearing agents is
recommended for CNS tissues?
a. Carbon tetrachloride
b. Aniline oil
c. Clove oil
d. Cedarwood oil
d. Cedarwood oil
Which of the following type of knife is used to cut resin embedded sections?
a. Glass knife
b. Ralph knife
c. Diamond knife
d. Steel knife
c. Diamond knife
Which of the following dehydrating agent is used in blood film preparation?
a. Methyl alcohol
b. Butyl alcohol
c. Denatured alcohol
d. Ethyl alcohol
a. Methyl alcohol
Which of the following chemical agent is used to measure the completeness of
decalcification process?
a. Calcium carbonate
b. Calcium carbide
c. Calcium oxalate
d. Triple phosphate
c. Calcium oxalate
Copper sulfate will turn to ____ color if the dehydration process is completed.
a. Green
b. Violet
c. Blue
d. White
d. White
The presence of an odor in the final bath of paraffin that smells like the _______ indicates that the paraffin wax in the baths should be changed.
a. Clearing agent
b. Fixative
c. Dehydrating agent
d. Decalcifying agent
a. Clearing agent
Which of the following is the most common and regarded as one of the best embedding media?
a. Gelatin
b. Celloidin
c. Plastic
d. Paraffin
d. Paraffin
Which of the following fixative is best used for iron containing granules tissue?
a. 10% Formol saline
b. Formol calcium
c. 10% Neutral buffered formalin
d. Formol corrosive
c. 10% Neutral buffered formalin
The biconcave knife is ideally used for tissue embedded using ___.
a. Gelatin
b. Celloidin
c. Paraffin
d. Resin
c. Paraffin
Which of the following is not a characteristic of an ideal dehydrating solution?
a. It should not be able to dehydrate
fatty tissues
b. It should not harden tissues
excessively
c. It should not remove stains
d. It should not evaporate very fast
a. It should not be able to dehydrate
fatty tissues
In vacuum embedding, a negative
atmospheric pressure is applied to facilitate the procedure in such way the machine has a manometer to monitor that the pressure is maintained at ______.
a. 400-500 mmHg
b. 300-600 mmH
c. 500-600 mmHg
d. 350-500 mmHg
a. 400-500 mmHg
Admixture of water with the paraffin may be resolved by bringing the heating temperature all the way up to ______.
a. 120-150°
b. 100-120°C
c. 100-105°C
d. 150-200°C
c. 100-105°C
Paraffin wax may be used for the second time granted it was not smelling like the agent used prior to embedding and if it was filtered using ___.
a. Greens No. 905
b. Greens No. 90
c. Greens No. 903
d. Greens No. 904
d. Greens No. 904
Which of the following decalcifying agent is ideal for surface decalcification?
a. Von Ebner’s
b. Tricholoroacetic acid
c. 5% Formic acid
d. Perenyi’s fluid
a. Von Ebner’s
What is the recommended size of the honing stone?
a. 3 inch x 5 inch
b. 8 inch x 2 inch
c. 8 inch x 5 inch
d. 8 inch x 3 inch
d. 8 inch x 3 inch
Most rapid fixing fixative
a. Bouin’s
b. Flemmings solution
c. Carnoy’s fluid
d. Newcomer’s fluid
c. Carnoy’s fluid
Working solution of formalin is made at what concentration?
a. 37%
b. 40%
c. 10%
d. 20%
c. 10%
Regaud’s fluid is ideal in the preservation and demonstration of the following cell organelle except.
a. Golgi bodies
b. Mitochondria
c. Endoplasmic reticulum
d. Chromatin
c. Endoplasmic reticulum
What would be the appropriate melting point of the paraffin if the current laboratory temperature is at 23°C.
a. 52°
b. 53°
c. 51°C
d. 55°C
d. 55°C
Which of the following process is incomplete is a tissue specimen submerged in xylene had turned milky?
a. Dealcoholization
b. Clearing
c. Fixation
d. Dehydration
d. Dehydration
Which of the solution may act as both fixative and a decalcifying agent?
a. 5% Formic acid
b. Perenyi’s fluid
c. Von Ebner’s
d. Phloroglucin nitric acid
a. 5% Formic acid
Which of the following is the coloring
substrate of hematoxylin?
a. Globin
b. Hematin
c. Heme
d. Hemoglobin
b. Hematin
Dehydrating agents are generally used in what order of concentration?
a. Either
b. Ascending
c. Neithe
d. Descending
b. Ascending
Make tissues clear but does not remove
alcohol.
a. Glycerine
b. Clove oil
c. Methyl benzoate
d. Aniline oil
a. Glycerine
Which of the following is regarded as the
most rapid clearing agent?
a. Chloroform
b. Toluene
c. Carbon tetrachloride
d. Xylol
d. Xylol
Sections to be cut using the ultrathin
microtome are embedded using ___.
a. Gelatin
b. Paraffin
c. Celloidin
d. Resin
d. Resin
Which of the following embedding medium is recommended for large tissue blocks such as the bones and the brain?
a. Embeddol
b. Carbowax
c. Paraplast
d. Ester wax
c. Paraplast
A plane wedge knife is ideally used using a ___.
a. Sliding microtome
b. Rocking microtome
c. Ultrathin microtome
d. Rotary microtome
a. Sliding microtome
Which of the following dehydrating agents is ideal for delicate tissue such as plant and animal tissue for it is regarded to have a slow penetrating action.
a. Butyl alcohol
b. Ethyl alcohol
c. Denatured alcohol
d. Methyl alcohol
a. Butyl alcohol
Which of the following honing stone gives the best result for honing a knife that had been blunted and nicked?
a. Arkansas
b. Fine carborandum
c. Whetston
d. Belgium yellow
d. Belgium yellow
Most sensitive way of measuring
completeness of the decalcification process.
a. Chemical method
b. None of these
c. Physical method
d. Xray method
d. Xray method
Fixative commonly used in the diagnosis of cases for rabies.
a. Acetone
b. Flemming’s solution
c. Osmium tetroxide
d. Tricholoroacetic acid
a. Acetone
Rossman’s formula for fixing tissue in frozen section contains ___?
a. Acetone
b. Alcohol
c. Formalin
d. Calcium
a. Acetone
Which of the following clearing agent is ideal for urgent biopsies?
a. Benzene
b. Xylene
c. Toluene
d. Chloroform
a. Benzene
Which of the following is not a service of the chemical xylene in the routine H&E staining process?
a. Dealcoholization
b. Differentiator
c. Deparaffinization
d. Clearing
b. Differentiator
Which of the following knife is utilized to
tough tissue specimen that is embedded
using paraffin?
a. Plane convex knife
b. Plane concave knife
c. Biconcave knife
d. Plane wedge knife
d. Plane wedge knife
Which of the following decalcifying agents is ideal for electron microscopy in tissue sections?
a. Acid decalcifying agents
b. Ion exchange resins
c. Electrophoresis
d. Chelating agents
d. Chelating agents
