Three Dimensional Structure And Function Flashcards
What is a space-filling model?
A space-filling model depicts each atom as a solid sphere, illustrating the dense, closely packed nature of folded polypeptide chains. It is used to show the overall shape of a protein and the surface exposed to aqueous solvent.
[PDB 1AFK]
What is a simplified cartoon model?
A simplified cartoon model emphasizes the backbone of the polypeptide chain, eliminating amino acid side chains. This model allows for easier visualization of how the polypeptide folds into a three-dimensional shape and reveals elements of secondary structure.
[PDB 1AFK]
What is a detailed model?
A detailed model emphasizes the structures of amino acid side chains, covalent bonds, and weak interactions between atoms. These models are important for understanding how a substrate binds in the active site of an enzyme.
[PDB 1AFK]
What is nuclear magnetic resonance (NMR) spectroscopy?
Nuclear magnetic resonance spectroscopy is a technique that allows for the study of proteins in solution without the need for crystal preparation. It involves placing a protein sample in a magnetic field and recording interactions between atoms.
[PDB 1AFK]
How are NMR structures compared to X-ray crystal structures?
NMR structures and X-ray crystal structures of proteins are compared to determine accuracy. In some cases, the structures may differ due to disordered regions that do not show up in X-ray crystal structures.
[PDB 2AAS]
