thomas - part 1 Flashcards

1
Q

how much of european population died in black death

A

30-60%

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2
Q

what did they think caused the black death

A

evil vapours - miasmata

bad air - mal aria around swamps

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3
Q

what causes the black death

A

bacteria yersnia pestis

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4
Q

who invented disease seeds

A

girolamo fracastoro

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5
Q

what was disease seeds the first step of

A

germ theory

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6
Q

what did antoni van leeuwenhoek find

A

animalcules

microorganisms

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7
Q

who thought airborn dust carried germs of yeast and microorganisms

A

louis pasteur

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8
Q

what was pasteurs central theory

A

the transformation of organic matter is effected by specific microorganisms with specific requirements for nutrients and temperature

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9
Q

what treatment is given to wine

A

55 degrees briefly then rapid cooling

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10
Q

time and temp for pasteurisation

A

72 degrees

15 seconds

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11
Q

what did koch find

A

bacillus anthracis causes anthrax
microorganisms responsible for tuberculosis and cholera
kochs postulates

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12
Q

what are kochs postulates

A

microorganisms found in abundance in organisms suffereing from a disease, not in healthy
microorganism isolated
then shown to cause disease alone
reisolated and shown to be the same

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13
Q

what did koch invent

A

crystal violet to visualise bacterial cells

human cells not stained

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14
Q

different types of bacteria koch found

A

gram positive and negative

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15
Q

average cell length and volume of bacteria

A

1 micrometer

1-2 micrometres squared

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16
Q

are bacteria pro or eukaryotic

A

prokaryotic

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17
Q

what are gram positive bacteria cell walls made of

A

peptidoglycan and teichoic acids

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18
Q

what is peptidoglycan made of

A

carbohydrates and amino acids

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19
Q

what is the cell wall of a gram negative bacteria made from

A

peptidoglycan
phospholipids
lipopolysaccharides

20
Q

best early antiseptic

21
Q

difference between antiseptic and disinfectants and antibiotic

A

antiseptic kill bacteria on surface of skin
disinfectants kill bacteria on non living objects
antibiotics kill bacteria within the body

22
Q

what antiseptics were used end of 19th century

A

chlorine releasing agents like chloramine T

23
Q

antiseptic by lister

24
Q

issue with phenol

A

blistering

25
best results in antibacterials
sulphonamides
26
how do sulphonamides work
disrupts dihydropteroate synthetase | stops tetrahydrofolate being made in bacteria not mammals
27
who invented penicillin
fleming
28
what was first US patient treated for with penicillin
septicaemia
29
how many doses were made for D day
2.3 million
30
who discovered structure of penicillin
dorothy hodgkin
31
main feature of penicillin
beta-lactam ring
32
how do beta lactam rings work
disrupts the formation of transpeptidase enzyme which joins peptidoglycan chains together
33
what is nucleophile
electron rich electron pair donator negative
34
what is electrophile
electron deficient, accepts lone pair | positive
35
steps to forming bacterial cell wall
hydrolysis of amide bond in peptide formation of ester with serine in enzyme's active site reaction of amine of adjacent chain to form amide
36
reaction of beta lactams
reacts with transpeptidase | reacts with side chain of amine acid ser in active site
37
other beta lactam
cephalosporins
38
how did they deduce the structure of cephalosporins
NMR
39
how did they make cephalosporin
converting penicillin
40
how does ciprofloxacin work
blocks DNA synthesis of gram negative bacteria
41
how does linezolid work
blocks bacterial protein synthesis
42
what causes toxic shock
release of toxins from staphylococcus aureus into blood
43
how are bacteria resistant to penicillin
penicillinase destroys beta lactam ring
44
how to stop beta lactamases from workking
add clavulinic acid
45
last resort antibiotics
carbapenems vancomycin colistin
46
what is vancomycin
glycopeptide IV administered severe side-affects disrupts cell wall formation
47
what is colistin
active against gram negative IV administered side affects disrupts cell wall displaces cations stabilising the LSP then disrupting membranes