Third Test 160404 Flashcards
What are the characteristics of Sound (and how are they measured)?
- amplitude or intensity (measured in decibels, dB)
- frequency or pitch (Hz or kHz or cycles per second)
- phase (used for direction of sound)
What is decibel?
Decibels (dB) = 20 x log10 (p1/p2)
Where p1 and p2 are pressures in dyne/cm2
Define ‘pure tone’
Sound with only one component
Many sounds have regular set of components: eg f, 3f, 5f etc.
What is the ‘fundamental’ frequency?
The lowest frequency
What is ‘harmonics’?
Sound of higher integer multiples
List components of the outer ear
- pinna (auricle or flap)
- auditory canal
Why is the Pinna important?
For sound localization
List the components of the middle ear
- eardrum (tympanic membrane)
- ossicles (malleus, incus and stapes)
Why is the Eustachian (auditory) tube important?
It keeps air pressure equal on both sides of the eardrum
Sounds waves in outer vs inner ear
Outer: in air
Inner: in fluid
The impendance (resistance to compression) of water is much higher than that of air.
Hence without middle ear, 97% of energy would be reflected, but with it only 40% is (so we can hear better)
List the components of the inner ear
- cochlea
- vestibular apparatus
List events of sound reception in inner ear
- sound travels down auditory canal and hits middle ear, the stapes moves like a piston
- pressure wave transmitted through oval window of cochlea
- pressure wave moves basilar membrane - cilia bend on hair cells
- graded potential occurs with release of neurotransmitter
- basilar membrane vibrates, tectorial membrane as well but in a different direction - causes bending of hair cells
- sound to oval window
- wave travels down basilar membrane and reaches maximum height at some point
Describe function of the basilar membrane
Is a mechanical frequency analyzer:
- each location along the membrane is tuned to different frequency due to varying stiffness
- high frequency peak near base (oval window)
- low frequency peak near apex (helicotrema)
Describe the innervation of the organ of Corti
- afferent nerve fibers arise from nerve cell bodies within spiral ganglion
- 95% contact inner hair cells (each of which consists of the sole terminus for up to 10 axons)
- 5% contact the outer hair cells
What are the hair cells innervated by (in the ear)?
The cochlear (auditory) nerve (branch of 8th CN - vestibulocochlear nerve) With neuron cell bodies in the spiral ganglion
Describe the action of the cochlear nerve fibers
They fire in a phase-locked manner
I.e. Fire at peaks of wave
Where does the cochlear nerve project to?
To the dorsal and ventral cochlear nucleus in the medulla
- making tonotopic maps here
Where does the ventral cochlear nucleus project to?
Superior olivary nucleus
- inputs from both ears are correlated here and sound source is localized
- it’s here that there’s a response to inter-aural differences and phase differences
Where does the dorsal cochlear nucleus project to?
Superior olivary nucleus
- with information about the response to sound pattern (e.g. Frequency modulation
Where does the superior olivary nuclei project to?
(Via lateral lemmiscus) to inferior colliculus in midbrain
- cells respond to amplitude and frequency modulation, inter-aural amplitude and phase differences, direction of sound motion in space
Where does the inferior colliculus project to?
Medial geniculate in thalamus
Describe sound localization
Each neuron on the lateral superior olive receives inputs from both ears
- ear located on the same side provides excitatory input to dendrites
- ear located on the opposite side provides an inhibitory input
Describe sound localization based on amplitude
Sound source in front - excitatory and inhibition is equal
Sound to the side - excitatory and inhibition is unequal
Describe the traveling wave theory (by Georg Von Bekesy
- a sound impulse sends a wave along the basilar membrane.
- as the wave moves along the membrane it’s amplitude increases until it reaches a maximum, then falls off sharply until the wave dies out
- where the wave reaches its greatest amplitude is the point at which the frequency of the sound is detected by the ear
- high frequency = base of cochlea
- low frequency = apex
ERG: what does the a-wave measure?
The general physiological health of the photoreceptors in the outer retina
ERG: what does the b-waves measure?
The health of the inner layers of the retina
- The ON bipolar cells
- the muller cells
ERG: what does the c-wave measure?
The pigment epithelium
ERG: what does the d-wave measure?
The activity of the OFF bipolar cells
ERG: what does the oscillitatory potentials reflect?
The activity in amacrine cells
Define electrooculogram (EOG)
Electrophysiological test of function of the outer retina and retinal pigment epithelium
- records change in the electrical potential between the cornea and the ocular fundus during successive periods of dark and light adaptation
- diagnostic method of the human oculomotor system
Define BEST’s disease
Sight loss can be variable but it threatens central vision in one or both eyes
What is Fundus photography?
Photograph of interior surface of the eye, including
- retina
- optic disc
- macula
- posterior pole
What is fundus photography used for?
Monitoring progression of a disease
Diagnosis of a disease (in combination with retinal angiography)
Describe the properties of a fundus camera
Typical camera views 30 to 50 degrees of retinal area, with magnification 2.5x
Allows modification through zoom or auxiliary lenses from 15 degrees (5x magnification) to 140 degrees with wide angle lens (which minifies image by half)
Define sound
A temporal modulation of air pressure
How does the fundus camera work?
Observation light is focused via a series of lenses through a donut shaped aperture, then passes through a central aperture from an annulus, before passing through the camera objective lens and through cornea onto the retina.
Light reflected from retina passes through the un-illuminated hole in the donut formed by the illumination system.
Minimal reflections are captured in the image because the light paths of the two systems are independent.
When button is pressed to take a photo, a mirror interrupts the path of illumination system allowing light from flashbulb to pass into the eye.
A mirror also falls in front of the observation telescope, redirecting reflected light onto the capturing medium
What is fundus autoflourescensce used for?
Documenting metabolic changes at the level of retinal pigment epithelium, suggesting areas of high risk for visual function loss
What causes fundus autoflourescensce?
The light-sensing molecules (retinoids) in the photoreceptors are succeptible to damage and cross-linking. Photoreceptors shed their damaged outer segments which the RPE ingests through phagocytosis. The molecules are stored in liposomes and form lipofuscin (LF).
Age and disease can contribute to a buildup of LF in RPE. LF is made into fluorescence by 500-800 nm wavelengths, they then release a photon of light which is recorded
Define hyperfluorescence
Areas of excess lipofuscin accumulation during fundus autoflourescensce will appear hyperfluorescence
Define hypofluorescence
When RPE cells die or are absent, lipofuscin disappears, leading to hypofluorescence
What does fluorescein angiography do?
Evaluates blood vessels in the eyes with macular or retinal disease.
Used to asses the integrity of the retinal and choroidal vasculature.
Can be used to detect diabetic retinopathy, occlusions, edoema, and tumours
Describe procedure of fluorescein angiography
Test requires dilation of the pupils and a small injection of vegetable dye (through an IV needle into a vein in your arm)
A series of pictures are taken in the space of 15-20 mins.
For most patients: no side effects
Some will feel nauseated for a few minutes
What information does an a-scan ultrasonography give you?
Information regarding the density of the tissues
Describe procedure of ophthalmic ultrasonography
Uses high-frequency sound waves, transmitted from a probe into the eye.
As the wave strike intraocular structures, they’re reflected back and converted into an electric signal, which is reconstructed as an image and can be used to make a dynamic evaluation of the eye
Describe an a-scan ultrasonography
A thin, parallel sound beam is emitted, passes through the eye and images one small axis of tissue
- the echoes are represented as spikes arising from a baseline.
- the stronger the echo, the higher the spike
Describe a b-scan ultrasonography
An oscillating sound beam is emitted, passing through the eye and imaging a slice of tissue
- the echoes are represented as a multitude of dots that together form an image
- the stronger the echo, the brighter the dot
What information can a b-scan ultrasonography tell you?
It can help you differentiate a posterior detachment (a benign condition) from a more highly reflective retinal detachment (a blinding condition)
What is OCT?
An imaging technique that allows a detailed view of living structures within the eye, non-ivasively
How does OCT work?
Similar to ultrasound b-scan
Uses reflectivity of light instead of sound to produce detailed images (tomograms)
Low-coherent near-infrared light beam split into two
- one beam to a reference mirror, other to the imaged tissue
- back scattered light from both surfaces combine, interference occur and an image is generated
What does OCT stand for?
Ocular coherence tomography
What is the resolution of an OCT?
High resolution 1-10 um
Define sensation
A conscious or unconscious awareness of external or internal stimuli
What is sensory input?
Sensory impulses reach the CNS and become part of a large pool of sensory input
Each piece of incoming information is combined with other arriving and previously stored information
What is sensory modality
The property by which one sensation is distinguished from another