Thermal Denaturation Profile for DNA

Question 1

what is the melting of DNA

Answer 1

the Denaturation of DNA

Question 2

what are the two strands held by

Answer 2

held together by weak, non-covalent bonds that readily separated and are induce experimentally using heat

Question 3

what is the process of DNA denaturation?

Answer 3

gradually increase the temperature and can be followed using spectrophotometry.

Question 4

what is the process of DNA renaturation?

Answer 4

reverse the process of Denaturation by lowering the temperature

Question 5

what happens during DNA denaturation when it is heated slowly?

Answer 5

DNA melts in a relative narrow temperature range
With and increase in absorbance at 260nm
Allow to cool and the strands re-associate

Question 6

what happens to complimentary strands during DNA renaturation?

Answer 6

collide randomly followed by rapid “zipping up” of adjacent nucleotide pairs

Question 7

what effects the time of DNA renaturation?

Answer 7

length and concentration of DNA