Melting Temperature of DNA Flashcards
1
Q
what is the melting temperature of DNA dependent on?
A
DNA base composition and length
2
Q
Do AT pairs take longer to melt or do GC base pairs? and Why?
A
GC bases are held together by 3 hydrogen bonds while AT bonds have 2 bonds, thus GC pairs have a higher melting temp.
3
Q
what also increase melting temperature?
A
proper pairing also increase melting temp. not all DNA have complimentary strands.
