The Assembly of Clatherin Coats Drives the Formation of Vesicles from the Plasma Membrane and TGN Flashcards
What does the binding of adaptor protein complexes to the plasma membrane require?
ATP and GTP
what appears to provide part of the driving force for formation of a vesicle?
the assembly of a clatherin coat on the cytosolic side of a membrane
initially, all clatherin units are…? which forms?
hexagonal and form a planar structure
what happens when more triskelions are incorporated into the growing lattice?
some pentagons are formed
what does the mixture of pentagons and hexagons allow?
allows the new coat to curve around the budding vesicle
what is required as clatherin accumulates around the budding vesicle?
dynamin is required for constricting/closing the vesicle
what is dynamin? what happens as GTP is hydrolyzed?
a closed cytolsolic GTPase; as GTP is hydrolyzed, dynamin rings tighten and separate the vesicle from the plasma membrane
Uncoating the vesicle: what happens once the vesicle is formed?
clathein dissociates readily
Uncoating the vesicle: what does uncoating require?
requires energy, provided by an uncoating ATPase
