Tests Flashcards

1
Q

BAP Agar

A

Blood
alpha hemolysis - green and partially hydrolysed
beta hemolysis - clear/yellow and comple hydrolysis
gamma hemolysis - none

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2
Q

MSA Agar

A
Mannitol salt (10%) inhibits all except staph spp.
Mannitol - fermented by staph, color change red to yellow
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3
Q

MacConkey

A

Selective and Differential: dyes inhibit gram pos, contains lactose so fermented changes to purple

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4
Q

Hektoen Agar

A

Contains lactose, thiosulfate, ferrous sulfate and green dye
If bact prod H2S, non lactose = green with black center
Salmonella spp.

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5
Q

ENT Agar

A

Contains bile (inhibs gram pos staph) and sodium azide (inhibs gram pos). Contains esculin and peptone as nutrients and ferric citrate as indicator.
Colonies are dark and spread
Enterococcus faecalis

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6
Q

MYC and SAB

A

Selective for fungi, acidic and anti-bact

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7
Q

Catalase

A

Tests if organism can detox some immune chems. (H202 to water)

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8
Q

Coagulase

A

interacts with prothrombin to clot

Staph aureus

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9
Q

Oxidase test

A

measures cytochrome oxidase presence. Pos = purple

Pseudomonas aeruginosa

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10
Q

Bile solubility test

A

if sensitive - clear liquid
strep pneumonia - sensitive
strep mitis - insensitive

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11
Q

Urease test

A

pink/red = pos; yellow = neg

Proteus spp and H. Pylori

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12
Q

Indole Test

A

Pos = red (dipstick=blue)

E. Coli

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13
Q

Sensitivity test

A

Optochin - strep pneu
Novobiocin - staph sapr
Bacitrian - strep pyogenes

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14
Q

PYR Test

A

Activity of pyrolidonyl arylamidase detected
Pos=pink
Group A strep (pyogenic), Group D Enterococci (Enterococcus spp), coag neg staph spp

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