Testing of donor blood

Question 1

immunohematologic testing

Answer 1

ABO forward & reverse
Rh
weak D if applicable
Ab screen

Question 2

if significant Ab is present in donor whole blood what can & cannot be transfused?

Answer 2

plasma cannot be transfused

RBC cells can be - need to be labeled

Question 3

Serologic test for syphilis

Answer 3

spirochetes do not survive in refrigerator temps but fresh blood & plts can transmit
ELISA against treponeme-specific antibodies to T. pallidum (screen & confirm)

Question 4

viral hepatitis tests:

Answer 4

Hep B surface antigen (HBsAg)
Ab to Hep B core (Anti-HBc)
Ab to hep C virus (Anti-HCV)
NAT (RT-PCR) to detect RNA of hep C

Question 5

HIV I/II & HTLV -I/II

Answer 5

NAT tests

reduced window period from 25 days to 5 days

Question 6

West Nile virus

Answer 6

mosquito borne disease that was introduced to blood supply
can recover
use NAT

Question 7

Cytomegalovirus (CMV)

Answer 7

most patients are carriers (80%) but asymptomatic

immunosuppressed patients & premature infants need CMV negative blood & leukoreduced blood

Question 8

Hemoglobin S testing

Answer 8

done for neonates & patients w/ sickle cell anemia