Testing for reducing sugars - Benedicts Test Flashcards
1
Q
Qualitative data
A
• non numerical
• difficult to standardise methods
2
Q
Standardise methods
A
Refers to standard deviation = statistical significance
3
Q
Redox
A
Reduction and oxidation
4
Q
OIL RIG
A
oxidation - lose of electrons
reduction - gain of electrons
5
Q
A substance is reducing
A
if it causes reduction of other substances
6
Q
Maltose and lactose
A
are reducing
7
Q
sucrose
A
not reducing
8
Q
Benedict’s reagent
A
mixture of sodium carbonate, sodium citrate and copper II sulphate
9
Q
carrying out the test
A
- 3cm cubed test solution is added to 3cm cubed of Benedict’s solution in a test tube
- tt placed in boiling water (70 degrees c or more) bath for between 3-5 minutes
10
Q
Colorimeter
A
Measures either absorbants or transmissions of light
11
Q
Dissociate
A
splitting
12
Q
Copper II sulphate dissociates to form
A
Cu2+ ions that are blue
13
Q
When reduced
A
Brick red Cu+ ions are formed
14
Q
More reducing agent there is
A
More Cu+ ions produced, less Cu2+ ions in solution
