ice cold, isotonic Flashcards
1
Q
Outline the role of organelles in the production, transport and release of
proteins from eukaryotic cells
A
DNA in nucleus is code (for protein);
2. Ribosomes produce (protein);
3. Mitochondria produce ATP (for protein synthesis);
4. Golgi apparatus packaged/modified
5. Vesicles transport
6. (Vesicles) fuse with cell membrane;
2
Q
tissue sample must be ice cold
A
reduce enzyme activity
3
Q
isotonic solution
A
Prevents osmosis so no lysis of
organelles
4
Q
buffered solution
A
prevent denaturing of enzyme