Testing

Question 1

What does a positive Catalase test look like?

Answer 1

Hydrogen peroxide production

Question 2

Catalase tests are most useful in distinguishing between ___ and ___

Answer 2

Staph (+)

strep (-)

Question 3

Coagulase tests are most useful in distinguishing between ___ and ___

Answer 3

Staph aureus (+)

Staph epidermidis (-)
Staph saprophyticus (-)

Question 4

What does a positive Citrate test look like?

Answer 4

Blue color in slant

Question 5

Citrate tests are most useful in distinguishing between ___ and ___

Answer 5

Klebsiella (+)
Salmonella (+)

Shigella (-)
Escherichia (-)

Question 6

What does a positive Urease test look like?

Answer 6

Hot pink slants

Question 7

Urease tests are most useful in distinguishing between ___ and ___

Answer 7

Proteus (+)

Salmonella (-)
Shigella (-)

Question 8

MacConkey agar is used to test ___.

What does a positive test look like?

Answer 8

Lactose fermentation

Reddish purple

Question 9

Lactose fermentation tests are most useful in distinguishing between ___ and ___

Answer 9

KnEE (+)
Klebsiella
Escherichia
Enterobacter

Sneaky SPPY (-)
Salmonella
Shigella
Proteus
Pseudomonas 
Yersinia

Question 10

H2S (black precipitate) production is a useful test for distinguishing between __ and ___

Answer 10

Salmonella (+)

Shigella (-)

Question 11

Optochin/P disk sensitivity vs resistance is useful for distinguishing between __ and ___

Answer 11

Strep pneumoniae (S)

Viridans Strep (R)

Question 12

Bacitracin/A disk sensitivity vs resistance is useful for distinguishing between __ and ___

Answer 12

Strep pyogenes (S)

Strep agalactiae (R)

Question 13

Hemolysis tests are useful in distinguishing between __ and ___

Answer 13

Strep and Enterococcus spp.

A-S. pneumoniae, viridans strep

B-S. pyogenes, S. agalactiae

G-Enterococcus spp.

Question 14

What does mannitol salt agar fermentation look like?

Answer 14

Positive turns it yellow

Question 15

Mannitol fermentation helps in distinguishing between __ and ___

Answer 15

Staph aureus (+)

Staph epidermidis (-)
Staph saprophyticus (-)

Question 16

What does a positive oxidase test look like?

Answer 16

Purple or dark color on dry slide

Question 17

Oxidase tests are useful for distinguishing between __ and ___

Answer 17

Pseudomonas (+)
Vibrio (+)
Neisseria (+)
Campylobacter (+)
Helicobacter (+)
Moraxella (+)

Acinetobacter (-)
Proteus (-)
Salmonella (-)
Shigella (-)