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Micro Summary - Bacteria > Preliminary ID And Properties > Flashcards

Preliminary ID And Properties Flashcards

1
Q

Staphylococcus aureus

2

A

Coagulase +

Ferments mannitol

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2
Q

Staphylococcus epidermidis

3

A

Coagulase -
Non hemolytic
Does NOT ferment mannitol

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3
Q

Staphylococcus saprophyticus

2

A

Coagulase -

Novobiocin resistant

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4
Q

Streptococcus pyogenes

5

A 
Chain of cocci
Beta hemolysis
Group A
PYR +
Bacitracin sensitive
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5
Q

Streptococcus agalactiae

5

A 
Chain of cocci
Beta hemolysis 
Group B
Bacitracin resistant 
CAMP +
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6
Q

Streptococcus pneumoniae

4

A

lancet shaped diplococci
Alpha hemolytic
Bile sensitive
Optochin sensitive

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7
Q

Enterococcus

4

A

Pairs/short chains of cocci
Gamma hemolytic
Bile resistant
Antibiotic resistant

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8
Q

Bacillus anthracis

5

A 
Non motile
Non hemolytic
String of pearls
Unique capsule
Medusa head colonies
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9
Q

Bacillus cereus

3

A

Motile
Hemolytic
Emetic and diarrheal toxins

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10
Q

Clostridium perfringens

5

A 
Box shaped
Anaerobic
Rapid growth
Many toxins 
Nagler reaction
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11
Q

Corynebacterium diphtheriae

2

A 
Pleomorphic rod (Chinese letters)
dark pharyngeal pseudomembrane
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12
Q

Listeria monocytogenes

3

A

Catalase +
Tumbling motility at room temp
Umbrella motility

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13
Q

Actinomyces israelii

3

A

Anaerobic
Molar tooth colonies
Sulfur granules

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14
Q

Nocardia spp

3

A

Weakly Acid fast
Slow growth
Fuzzy colonies (aerial hyphae)

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15
Q

Mycobacterium tuberculosis

4

A

Strongly acid fast
Slow growth
non pigmented colonies
Mantoux and Quantiferon tests

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16
Q

Neisseria meningitidis

4

A

Diplococci
Fastidious
Can grow on chocolate agar
Gram stain blood/CSF before antibiotics

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17
Q

Neisseria gonorrhoeae

4

A

Diplococci
In PMN cells in urethritis specimen
Fastidious
Grows on Thayer Martin agar

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18
Q

Moraxella catarrhalis

2

A

Diplococci

Grows on chocolate and blood agar

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19
Q

Acinetobacter spp

3

A

Very small rods in pairs
Oxidase -
Grows on MacConkey

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20
Q

Enterobacteriaceae

2

A

Oxidase -

Grows on MacConkey

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21
Q

Enterobacteriaceae
Lactose fermenters
2

A

E. coli

Klebsiella

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22
Q

Enterobacteriaceae
Lactose non-fermenters
4

A

Salmonella
Shigella
Yersinia
Proteus

Plus pseudomonas (which is not enterobacteriaceae)

23
Q

Enterobacteriaceae
Citrate positive
1

A

Klebsiella

24
Q

Enterobacteriaceae
Urease positive
2

A

Klebsiella

Proteus

25
Q

Enterobacteriaceae
H2S producer
1

A

Salmonella

26
Q

Bacteroides, Fusobacterium

2

A

Weakly staining

Anaerobic

27
Q

Pseudomonas aeruginosa

5

A 
Oxidase +
Green 
Grape like smell
Growth on MacConkey
Lactose non fermenter
28
Q

Vibrio cholerae

A

Ferments sucrose

Thiosulfate citrate bile salt sucrose (TCBS) agar-yellow colonies

29
Q

Helicobacter pylori

Test

A

Urea breath test

30
Q

Campylobacter spp

2

A

Thin, S shaped rod

Growth on media with antibiotics at 42 degrees C

31
Q

Haemophilus spp

3

A

Small coccobacilli
X and V factor

Satellite phenomenon (growth near Staph aureus on blood agar)

32
Q

Legionella spp

3

A

Weak staining
Buffered charcoal yeast extract agar (BCYE)
Needs cysteine to grow

33
Q

Bordetella pertussis

3

A

Fastidious
Regan Lowe charcoal agar
Use Dacron swabs (no cotton)

34
Q 
Yersinia pestis
Looks like (2)
A

Safety pin appearance

Fried egg colonies

35
Q

Brucella spp

2

A

Very small coccobacilli

Slow growth

36
Q

Francisella spp

3

A

Very small coccobacilli
Slow growth
Biohazard

37
Q

Treponema pallidum

Testing

A

If positive RPR and VDRL, confirm by FTA-ABS or MHA-TP

38
Q

Borrelia burgdorferi

Dx by__

39
Q

Borrelia hermsii

Serology?

A

Serology is useless

40
Q

Leptospira spp
2
Shape
Dx

A

Hook shaped

Dx by serology

41
Q

Rickettsia spp
3
Tests

A

Poorly staining
Fluorescent antibody test
Weil-Felix test

42
Q

Erlichia and Anaplasma
3
Tests

A

Poorly staining
PCR
serology

43
Q

Coxiella burnetti
2
Tests

A

PCR

Serology

44
Q

Chlamydia species

3

A

No cell wall
NAAT test
Inclusion bodies

45
Q

Mycoplasma species 5

A 
No cell wall
PCR
Eaton's agar
Mulberry shaped (M. pneumoniae)
Fried egg shape (all others)
46
Q

All strep are catalase __

47
Q

All staph are catalase __

48
Q

All enterococcus and enterobacteriaceae are catalase ___

Enterobacteriaceae are oxidase ___

A

Negative

Negative

49
Q

Urease positive

A

PUNCH K

Proteus
Ureaplasma
Nocardia
Corynebacterium 
Helicobacter 
Klebsiella
50
Q

Oxidase positive

A

PVNCH

Pseudomonas 
Vibrio
Neisseria
Campylobacter
Helicobacter
51
Q

Urease negative

Key bacteria

A

Salmonella

Shigella

52
Q

Oxidase negative

Key bacteria

A

Salmonella
Shigella
Proteus

53
Q

H2S producer

A

Salmonella

Micro Summary - Bacteria (8 decks)

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