TEST FOR SECONDARY HEMOSTASIS Flashcards
Tests for secondary hemostasis:
CPA-STRDS
Clotting time
prothrombin time
activated partial thromboplastin time
stypven time
thrombin time
Reptilase time
Duckert’s Test
Substitution studies
Measures Period Required for FREE FORMATION OF BLOOD TO CLOT after it has BEEN REMOVED FROM THE BODY
screen for problems in the blood clotting/coagulation mechanism
Clotting time
2 methods of clotting time
2methods of capillary blood method
capilliary blood method
-slide drop
-dale and laidlaw’s method
lee and white method
normal value for slide drop method
2-4 mins
normal value for lee and white method
7-15 mins
monitors the coagulation factors of the extrinsic and common pathway
used to monitor coumarin/coumadin/warfarin
Prothrombin time
Normal Value for PT
10-13 seconds
specimen for PT
PPP + thromboplastin + CaCl2
standardized way of reporting PT (INR)
International Normalized Ratio
Monitors the coagulation factors of Intrinsic and Common pathway
used to monitor heparin therapy
activated partial thromboplastin time
normal value for aptt
<35 seconds
specimen for aptt
PPP + appt reagent
reagents for appt
Activator: (micronized silica, ellagic acid, cellite, kaolin)
phospholipid
CaCl2
Used to detect deficiencies in fibrinogen
prothrombin
factor V and X
detects coagulation deficiencies in the common pathway
Stypven Time (russel viper venom time)
normal value for Stypven time
6-10 seconds
reagent for stypven time
east indian viper (Viperarusselli)
detects fibrinogen deficiency
sensitive test in detecting heparin inhibition
Thrombin time
thrombin time is prolonged in?
(FPPP)
Fibrinogen deficiency
presence of thrombolytic agent
presence of FDP and FSP
presence of heparin
normal value for thrombin time
10-14 seconds
specimen for thrombin time?
PPP + thrombin
detects fibrinogen deficiency uses reptilase
reptilase time
Reptilase time is prolonged in? (FPP)
Fibrinogen deficiency
Presence of thrombolytic agent
Presence of FDP and FSP
normal value for reptilase time
10-15 seconds
detects factor 13/XIII deficiency
Duckert’s test/5M urea solubility test
Reagents of Duckerts Test
5M urea
1% monochloroacetic acid
2% acetic acid
positive for Factor 13 deficiency in duckert’s test shows?
solubility to 5M urea at RT
used to identify individual factor deficiency by mixing correction reagents with patient’s plasma and then performing PT and aPTT
Substitution studies/Mixing Studies
