FIBRINOLYSIS TEST Flashcards
tests for fibrinolysis:
WEPEL
Whole blood clot lysis time
Euglobin Clot lysis Time
Protamine sulfate test
Ethanol gelation Test
Latex D-dimer Test
clot should remain intact for approximately 48 hours after incubating at 37 C
Whole blood clot lysis time
time for increased fibrinolysis in WBCLT
<48 hours
these are proteins that precipetate when plasma is diluted with water and acidified
euglobulin
A laboratory test that measures the time it takes for a clot, formed from the euglobulin fraction of plasma, to dissolve. It assesses the efficiency of the body’s fibrinolytic system.
Euglobulin Clot lysis Time
Euglobulin consists of:
Plasminogen
Plasmin
Fibrinogen
Fibrinogen Activators
what is needed to precipetate euglobulin
Plasma + Acetic acid
what is needed to clot euglobulin
Euglobilin dissolve in buffer + thrombin
time for euglobin clot lysis time that shows increased fibrinolysis
<2 hours
Detects the presence of Fibrinogen Monomers
Protamine sulfate test
Principle for protamine sulfate test
Plasma + Protamine sulfate = paracoagulation (gel-like clot)
Abnormal result for protamine sulfate test
Gel formation
Less sensitive test but more specific test than protamine sulfate
detects the presence of fibrinogen monomers
ethanol gelation test
Reagents for Ethanol gelation test
Plasma
Ethanol
NaOH
Abnormal Result for ethanol gelation test
Gel formation
used to evaluation of specific fragments ______
to monitor the fibrinolytic pathway is functional
- D-dimer
- latex D-dimer test
Principle of Latex D-dimer test
Based on Enzyme Immunoassay
Summary of tests for fibrinolysis:
Primary and secondary
WBCLT
EUGLOBULIN CLOT LYSIS
PROTAMINE SULFATE
ETHANOL GELATION TEST
LATEX D DIMER TEST
WBCLT: <48
EUGLOBULIN CLOT LYSIS: <2hrs
PROTAMINE SULFATE: -/+
ETHANOL GELATION TEST: -/+
LATEX D DIMER TEST: -/+
