TEST FOR PRIMARY HEMOSTASIS Flashcards

1
Q

term used to describe the
mechanism of certain systems that keeps blood in the circulation and produces of clot in cases of blood vessel injury

A

HEMOSTASIS

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2
Q

The systems composing the process of hemostasis are the:

A

Blood vessels
Platelets
Coagulation factors
Fibrinolysis components

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3
Q

General Considerations in preventing premature activation activation

A

Contamination with Tissue
thromboplastin
Inappropriate container
Improper Temperature
Hemolysis

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4
Q

EQUIPMENT AND ANTICOAGULANT

A

Needle Size
Evacuated Tubes/Syringes

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5
Q

NEEDLE SIZE:
_______most commonly
used for coagulation samples
_______collection of more
than 20 mL of blood
_______narrow or small
veins

A

20-gauge needle
19-gauge needle
21-gauge needles

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6
Q

Syringes and evacuated tubes should be______

A

SILICONE COATED.

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7
Q

ANTICOAGULANTS:

A

POTASSIUM CHLORIDE
TRISODIUM CITRATE
EDTA
HEPARIN

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8
Q

preferred anticoagulant
good in preserving the
labile factors______ and _____

A

TRI-SODIUM CITRATE.
Factor V and VIII

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9
Q

blood-to-anticoagulant ratio
for citrate

A

9:1

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10
Q

BLOOD COLLECTION:
1. _______is acceptable if tissue juice contamination must
be avoided
2. In anticipation of difficulties while doing venipuncture or if blood is being drawn for special procedures, using a ______ is recommended

A
  1. VACUUM TUBE METHOD
  2. TWO-SYRINGE METHOD
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11
Q

TEST FOR PRIMARY HEMOSTASIS

A

Bleeding time
Capillary resistance Test
Clotting Retraction Time
Platelet Count
Platelet Aggregation
Platelet Adhesiveness

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12
Q

actual time it takes for a standard wound to stop bleeding

A

BLEEDING TIME

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13
Q

BLEEDING TIME DETECTS:

A

Abnormalities of platelet number and function
Von Willebrand Factor deficiency
Abnormalities of vessel wall structure

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14
Q

BLEEDING TIME METHODS

A

MODIFIED DUKE METHOD
IVY METHOD

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15
Q
A
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16
Q

Detects abnormality of the capillaries due to a structural weakness in the capillary walls or thrombocytopenia

A

CAPILLARY RESISTANCE TEST

17
Q

CAPILLARY RESISTANCE TEST METHODS:

A

POSITIVE PRESSURE TEST
NEGATIVE PRESSURE TEST

18
Q

5 mL of whole blood is incubated at 37 deg Celsius for 1 hr

Clot will begin to shrink and retract from the walls of the tube

A

CLOT RETRACTION TIME/ MCFARLANE CLOT RETRACTION TECHNIQUE

19
Q

NORMAL VALUE FOR CLOT RETRACTION TIME

A

44-67%

20
Q

FORMULA FOR CLOT RETRACTION TIME

A

%CRT = VOL SERUM/TOTAL VOLUME OF WHOLE BLOOD X 100

21
Q

FACTORS OF CLOT RETRACTION TIME

A

1.NUMBER OF CONTRACTIBLE PLTS
2.PRESENCE OF Ca and ATP
3.normal concentration of fibrinogen

22
Q

PLT count methods

A

DIRECT & PLT ESTIMATION OF PBS

23
Q

Direct methods for plt count

A

TONKANTIN METHODS
BRECKER-CRONKITE METHOD
UNOPETTE METHOD

24
Q

Aggregation agents are added to PRP to induce shape change and aggregation of platelets

A

PLATELET AGGREGATION

25
Q

PLATELET AGGREGATION:
AGGREGATION AGENTS?

A

Epinephrine
Collagen
ADP
Ristocetin

26
Q

When blood is passed through a glass bead column, normal platelets that have VWF will adhere and aggregate to the beads

A

GLASS BEAD TEST

27
Q

FORMULA FOR PLT ADHESIVENESS

A

PA=(PC1-PC2/PC1)X 100%

28
Q

NORMAL VALUE FOR PLT ADHESIVENESS

A

70% or greater

29
Q

REFERENCE METHOD for
manual COUNT FOR PLT

A

BRECKER-CRONKITE METHOD