Test 5 Qs 1-10

Question 1

The direct IFA is used for?

Answer 1

Determination of antigen in a cell or tissue by direct labelling with antibodies labelled with FITC.

Question 2

To develop a test to screen circulating anti-brucella antibodies by immunofluorecence, you need all of the following EXCEPT:

a. A fluorescence microscope.
b. A positive serum control, know to contain anti-brucella antibodies.
c. A suspension of formalin-killed Bruscella melitensis.
d. Fluorescein-labelled anti-bovine IgG.
e. Isolated patient’s IgG labelled with a fluorescein compound.

Answer 2

e. Isolated patient’s IgG labelled with a fluorescein compound.

Question 3

FACS is used for?

Answer 3

Analysis and sort a mixture of the cells based on their differential ability to bind fluorescence-dye labelled ATB.

Question 4

Intensity of fluorescence in flow cytometer is detected by?

Answer 4

The PMT ( Pasteurella multocida toxin)

Question 5

CD Characteristic of cytotoxic T cells is?

Answer 5

CD8 (usually composed of one CD8ª(alpha) and one CD8ɓ(beta) chain.

Question 6

Which definition best describes lymphocytes?

a. Lymphocytes are leukocytes.
b. Lymphocytes are present in tissues.
c. Lymphocytes are cells that specialise in the production of cytokines.
d. Lymphocytes are antigen specific.
e. Lymphocytes can proliferate.

Answer 6

d. Lymphocytes are antigen specific.