Test 3 Qs 1-9

Question 1

Which of the following components is NOT substrate for enzymoimmunoassays?

a. TMB
b. BCIP / NBT
c. Fluorescein
d. DAB

Answer 1

c. Fluorescein

Question 2

Which of the following components is for IFA:

a. Antibody labelled with fluorochrome
b. Antibody labelled with peroxidase
c. Antibody labelled with alkaline phosphatase
d. Antibody labelled with radioisotope

Answer 2

a. Antibody labelled with flurochrome

Question 3

The indirect IFA is most commonly used for?

Answer 3

Testing of aitoimmune disorders.

Question 4

Flow cytometry is used for?

Answer 4

Analysis of the cells based on their differential ability to bind fluorwscence labelled antibody

Question 5

Sensitized erythrocytes are?

Answer 5

Erythrocytes labelled antibody (amboceptor)

Question 6

For testing of complement activation is NOT used:

a. Amboceptor
b. Patientˋs serum
c. Enzyme labelled antibodies specific to complement
d. Sensitised erythrocytes

Answer 6

c. Enzyme labelled antibodies specific to complement

Question 7

Complement fixation test is used for?

Answer 7

Only for determination of antigen or antibody presence

Question 8

In complement fixation test is positive result?

Answer 8

Inhibition of haemolysis of sensitised erythrocytes