Test 1 Qs 11-21 + abbreviations

Question 1

How long is blood incubated with distilled water to get hypotonic solution?

Answer 1

35-40 seconds

Question 2

Compliment fixation test. What is detected?

Answer 2

If patient has antibodies specific to Ag.

Question 3

In separation of leucocytes. What is the proportion between blood, distilled water and 2,7% NaCl?

Answer 3

1:2:1

Question 4

Which of the following markers are present in B-lymphocyte?

a. CD3
b. CD56
c. CD16
d. CD19

Answer 4

CD19

Question 5

In cell separating solution, what cells are separated first?

Answer 5

Lymphocytes and monocytes (Mononuclear cells)

Question 6

Index of phagocytic activity contains one information?

Answer 6

Number of particles in 100 phagocyte divided by hundred. But index must be over 5.

Question 7

Colorimetric test. what cell is removed first?

Answer 7

Red blood cell.

Question 8

What is the number of leukocytes in burks chamber if 88 leukocytes were counted in 50 squares. in 1 ml.

Answer 8

88 x 10^5 leuk / ml = 8 800 000

Question 9

(Draw a monocytes and if it has granules,) then what color would it get by Pappenheim staining?

Answer 9

It wouldnt stain because it is an granulocyte. this is a trick question.

Question 10

Fill in the gaps:

MHC II always expresses by ____________ and always expresses ____________ antigen.
__________proteins are present in cytosol of cell and ____________.

Answer 10

MHC II always expresses by APC (antigen-presenting cells) and always expresses processed antigen.
(Cbl) Cytoplasmic proteins are present in cytosol of cell and cell bodies.

(this can be wrong)

Question 11

What does NBT stand for?

Answer 11

NBT = Nitro blue nitrozolium

Question 12

What does INT stand for?

Answer 12

INT = Iodonitrotetrazolium test

Question 13

What does NOX stand for?

Answer 13

NOX = NADPH oxidase

Question 14

What does IMA stand for?

Answer 14

IMA = Index of metabolic activity.