Test 3 Flashcards
Lesion studies
Brain is damaged, animal is studied
Experimental ablation
Destroy part of brain and evaluate animal behavior
Excitotoxic lesions
Intracerebral injection of excitatory amino acid
Sham lesions
Placebo lesion
Stereotaxic surgery
How to insert electrode
Bregma
Junction between coronal and sagittal sutures after gap has closed
Stereotaxic atlas
Collection of drawings of sections in the brain
Stereotaxic apparatus
Device that helps scientists insert electrode into specific part of brain
Fixative
Prepare/preserve body tissue
Formalin
Most commonly used fixative
Microtome
Slices very thin body tissue
Scanning electron microscope
Provides 3d info about objects, doesn’t microscope as well as regular
Anterograde labeling method
Traces efferent axons
PHA-L
Anterograde tracer
Immunocytochemical methods
Indicates presence of chains of peptides with radioactive substances
Retrograde modeling method
Labels cell bodies that give rise to terminal buttons that form synapses with cells
Fluorogold
Retrograde tracer
Transneural tracing methods
Identifies series of neurons that form serial synaptic connections with each other
Computerized tomography (CT)
Produces 2d slice through body
Magnetic resource imaging (MRI)
Images interior of body
Diffusion tensor imaging (DTI)
Shows bundles of axons
Micro electrode
Records activity of individual neurons
Single unit recording
Records micro electrode
Macro electrode
Records activity of large numbers of neurons
Electroencephalogram (EEG)
Electrical brain potential
Magnetoencephalography
Magnetic field to detect groups of synchronized neurons
2-deoxyglucose (2-DG)
Sugar that enters cell but is not metabolized
Auto radiography
Locates radioactive substances in tissue
FOS
Protein produced in the nucleus of a neuron in response to synaptic stimulation
Functional imaging
Computerized method of detecting metabolic or chemical changes within the brain
Positron emission tomography (PET)
Reveals location of radioactive tracer in living brain
FMRI
Permits measurement of regional metabolism in the brain
Optogenetic methods
Stimulate/inhibit particular types of neurons
Transcranial magnetic stimulation (TMS)
Stimulation of brain, interferes with region being stimulated
Micro dialysis
Analyze chemicals in interstitial fluid
Genome
DNA
Allele
Different versions of genes
Targeted mutations
Mutated genes created in a lab and inserts into chromosomes
Antisense oligonucleotides
Modified strand of DNA/RNA that binds with specific molecules of messenger RNA
Sensory receptor
Specialized neuron that detects particular category of physical events
Sensory transduction
Sensory stimuli transduced into slow grades receptor potentials
Receptor potential
Slow grades electrical potential in response to physical stimulus. Strength affects firing rate
Electromagnetic spectrum
Detect presence of light