Test 3 Flashcards
Lesion studies
Brain is damaged, animal is studied
Experimental ablation
Destroy part of brain and evaluate animal behavior
Excitotoxic lesions
Intracerebral injection of excitatory amino acid
Sham lesions
Placebo lesion
Stereotaxic surgery
How to insert electrode
Bregma
Junction between coronal and sagittal sutures after gap has closed
Stereotaxic atlas
Collection of drawings of sections in the brain
Stereotaxic apparatus
Device that helps scientists insert electrode into specific part of brain
Fixative
Prepare/preserve body tissue
Formalin
Most commonly used fixative
Microtome
Slices very thin body tissue
Scanning electron microscope
Provides 3d info about objects, doesn’t microscope as well as regular
Anterograde labeling method
Traces efferent axons
PHA-L
Anterograde tracer
Immunocytochemical methods
Indicates presence of chains of peptides with radioactive substances
Retrograde modeling method
Labels cell bodies that give rise to terminal buttons that form synapses with cells
Fluorogold
Retrograde tracer
Transneural tracing methods
Identifies series of neurons that form serial synaptic connections with each other
Computerized tomography (CT)
Produces 2d slice through body
Magnetic resource imaging (MRI)
Images interior of body
Diffusion tensor imaging (DTI)
Shows bundles of axons
Micro electrode
Records activity of individual neurons
Single unit recording
Records micro electrode
Macro electrode
Records activity of large numbers of neurons