test 2 Flashcards
How are sperm cells specialised for their function?
1 - nucleus containing haploid chromosomes
2 - tightly packed mitochondria to provide ATP for movement of tail
3 - flagellum propels sperm
4 - microtubules keep sperm in suspension to move tail
5 - acrosome stores enzymes to digest zona pellucida
Describe the structure of an egg cell
1 - follicle cells release chem for acrosome reaction
2 - zona pellucida: glycoprotein for sperm to bind
3 - cortical granules
4 - lipid droplets: food store for embryo
What is the role of the cortical granules?
lysosome which releases enzymes to thicken zona pellucida and prevent further entry of sperm
Describe the stages of fertilisation
1 - Follicle cell releases chemical which triggers acrosome reaction
2 - Acrosome membrane fuses with front of sperm cell membrane and digestive enzymes released
3 - Enzymes digest zona pellucida
4 - Sperm membrane fuses with ovum membrane & nucleus enters ovum
5 - Cortical reaction: zona pellucida thickens to prevent entry of other sperm
6 - Egg and sperm nuclei fuse - diploid zygote
How does meiosis create genetic variation?
Crossing over of chromatids: same genes but different alleles
Independent assortment: different combo of maternal and paternal chromosomes
What is a gene locus?
Location of genes on a chromosome
What is gene linkage?
Genes with loci on the same chromosome so they aren’t inherited separately
The closer the loci of 2 genes…
The more closely they’re linked and less likely to be separated by crossing over
How do cells specialise?
Gene activated by stimulus
mRNA transcribed from active gene and translated into proteins
Protein determines structure and function of cell
How do transcription factors control gene expression in eukaryotes?
Methylation of DNA prevents transcription by stopping RNA polymerase binding
Histone modification - acetylation makes chromatin less condensed so RNA polymerase can bind
How can gene expression be controlled?
Rate of transcription of genes
Transcription factors
Activators & repressors
What is an operon?
Section of DNA containing promoter and operator
What happens when lactose is present?
Binds to repressor, changing its shape so it can’t bind to the promoter, B galactosidase expressed
What happens when lactose is absent?
Lac repressor binds to operator site and stops transcription as RNA polymerase can’t bind to promoter
What is biodiversity?
Number of different species and genetic variation within species
Define ecosystem
A self-sustaining life-supporting environment with biotic and abiotic factors
What is species evenness?
when species have similar abundances, no dominant species
How is genetic variation measured?
- DNA sequenced to determine bases and alleles
- DNA copied using PCR and cut with restriction enzymes
- fragments separated by gel electrophoresis to create a banding pattern
What does 2 bands in gel electrophoresis mean?
heterozygous as there’s a band for each allele
What is the heterozygosity index?
number of heterozygotes / number of individuals in a population
Why is a large heterozygosity index good?
more heterozygous a population, the larger the gene pool
increased chance of survival
Why is inbreeding bad?
heterozygosity lost, smaller gene pool
decreased chance of survival
recessive alleles have harmful effects (inbreeding depression)
How is maltose formed?
2 alpha glucose in condensation
How is sucrose formed?
Alpha glucose and alpha fructose (1-2) glycosidic bond
How is lactose formed?
Beta galactose and alpha glucose B(1-4) glycosidic
Function of maltose
Respiratory substrate, germinating seeds
Function of sucrose
Transporting energy in plants
Function of lactose
mammalian milk
How is the structure of starch related to its function?
Amylopectin large chain of glucose units: available for respiration
Amylose helix coiled: compact, lots of glucose in small space
Amylopectin is branched and compact: lots of glucose for resp
Large so it’s insoluble
How is the structure of glycogen related to its function?
Branched: easily hydrolysed do glucose released quickly
Compact, stores lots of glucose
Large, so it’s insoluble
Describe the structure of cellulose
Alpha glucose and beta
Unbranched straight chains align parallel to each other forming microfibrils (H bond)
What are some natural causes of rising temperatures?
- variations in solar activity
- fluctuations in ocean currents
- volcanic eruptions
How do ice cores help determine temperature?
measurement of O-18 and O-16
warmer climates have more O-18 because it is preferred in precipitation and evaporates
What are the conditions in peat bogs?
acidic, cool, anaerobic
Why do anaerobic conditions reduce decompositions?
no oxygen so decomposing bacteria can’t respire so die
digestive enzymes not present
acidic pH reduces enzyme action