Test 1 Flashcards
What is the role of the nucleus?
Contain genetic info as chromatin
Describe the structure of the nucleus
double membrane
nuclear pores
nucleolus
What is the nucleolus?
Dense area where ribosomes are made
What is chromatin?
DNA wrapped around histones
Describe the structure of mitochondria
Double membrane
Inner membrane folded into cristae
Matrix
What is the role of smooth ER?
Synthesis of lipid and hormones
What is the role of rough ER?
Synthesis of membrane bound or secreted proteins
What is the role of Golgi?
Modification and packaging of proteins
Describe the secretory pathway
1 - rER: Aa’s to proteins, folded to form tertiary structure & glycosylation
2 - packaged into vesicles and transported to Golgi
3 - Golgi: vesicles fuses & proteins modified
4 - proteins packaged into secretory vesicles and fuse with cell surface membrane
Which 8 organelles are found in prokaryotes?
Circular DNA, cell wall, pili, flagellum, food granules, mesosome, plasmid, capsule
Differences between prokaryotes and eukaryotes (4)
1 - prokaryotes have no nucleus
2 - P have circular DNA, E have linear
3 - P have 70s ribosome , E have 80s
4 - P non membrane bound
How does a protein’s secondary structure form?
Hydrogen bonds between peptides
What is a beta pleated sheet?
Polypeptide chains zig zag forming sheet of antiparallel strands
How do tertiary structures fold?
Bonds between R groups on amino acids
Describe the structure of a cell membrane
- phospholipid bilayer
- selectively permeable
- hydrophilic phosphate head on outside, hydrophobic fatty acid inside
- transmembrane, integral and peripheral proteins
- glycoproteins & glycolipids
- cholesterol
How can membrane fluidity be increased?
Unsaturated fatty acids so phospholipids less tightly packed
How can membrane fluidity by decreased?
More cholesterol
What is facilitated diffusion?
High conc to low conc via carrier or channel protein across a membrane
What molecules use a carrier protein?
Large molecules such as glucose and amino acids
What molecules use channel proteins?
Polar molecules such as ions
What is osmosis?
Net movement of water from high water pot to low water pot across a selectively permeable membrane
What is active transport?
Movement from low conc to high conc via carrier proteins across a membrane using energy from ATP
What is bulk transport?
Energy dependent transport of molecules in and out of cells
Compare globular and fibrous proteins
1 - chain of AA’s held by peptide bonds
2 - secondary structure held by hydrogen bonds
Contrast globular and fibrous proteins
1 - Globular has tertiary structure, fibrous doesn’t
2 - G has hydrophilic groups outside, F has hydrophobic groups outside
3 - G is spherical and compact, F is long chains
What happens in the G1 phase?
Main growth, high metabolic activity depending on nutrient supply and temp
What happens in the S phase?
Cell replication and repair
What happens in the M phase?
Cell divides and 2 genetically identical diploid cells produced
Why is mitosis needed?
Growth (new cells) and repair (replacing)
What is the estimated average requirement? (EAR)
energy intake which meets needs of 50% of people
What is the lower reference nutrient intake (LRNI)?
energy intake which meets needs of only 2.5% of people
What is the higher reference nutrient intake? (HRNI)
energy intake which meets needs of 97.5% of people (GDA)
What is SDA (specific dynamic action)?
energy expended for metabolising food (eating, digestion and absorption)
Describe atherosclerosis
1 - endothelial damage due to high Bp/toxins
2 - inflammatory response: WBCs deposit cholesterol, forming atheroma
3 - Ca2+ and fibrous tissue build up forming plaque
4 - RAISED BP as artery narrows
Why does atherosclerosis lead to CVD?
Reduced flow to cardiac muscle
increased risk of blood clots, blocking O2 supply
How does blood clotting lead to CVD?
Plaque in vessel ruptures, clotting occurs when thromboplastin is released by platelets
Blood supply is blocked
Heart is ischaemic
How do antioxidants prevent CVD?
prevent oxidation of LDL by providing electrons for free radicals