Test 2 Flashcards
Second exam
viruses that infect bacteria
bacteriophage
three main shapes of bacteria
spherical (cocci), rod-shaped (bacillus), spiral shaped (spirilla)
bacteria make up the kingdom ______
Prokaryotae
Prokaryotic cells contain ______
ribosomes
Prokaryotic cells do not contain _______
No membrane-bound organelles. So, no nuclei, mitochondria, chloroplasts, endoplasmic reticulum, golgi complex, lysosomes
Prokaryotic cells are surrounded by two things: _______ and ______
cell membrane (plasma membrane) and cell wall
Bacterial cell walls are composed of _______
peptidoglycan
______ is in both Gram positive and Gram negative cell walls
peptidoglycan
Gram positive bacteria stain this color
purple, because they retain the crystal violet stain
Gram negative bacteria stain this color
pink, because they don’t retain the stain
Gram ______________ have a thick peptidoglycan layer
Positive
Gram _______ have two layers (thin peptidoglycan and thick outer membrane of carbohydrates bonded to lipids
Negative
The antibiotic ______ interferes with peptidoglycan synthesis, therefore it works best on Gram _______ cells
penicillin, positive
Some bacteria produce a ______ or slime layer that surrounds the cell and protects them during infection in a human body
capsule
Four modes of motility of bacteria
Flagella, internal flagella (corkscrew), external slime, movement due to external stimuli (chemotaxis, phototaxis)
How many protein parts make up a bacterial flagellum
40 protein parts coated for by its own gene in order to create a working flagellum.
The three parts of a bacterial flagellum
Basal Body (produces the rotary motion), hook (connects it to the hollow filament), and the filament (several protein chains twisted into a helical structure)
Describe the structure of a Eukaryotic flagellum
Slender, cylindrical stalk covered by an extension of the plasma membrane. The core of the stalk contains a group of microtubules arranged so that there are nine attached pairs of tubules around the circumference and two unpaired microtubules in the center. (9 + 2)
At the base of the stalk is the basal body, which has nine sets of 3 microtubules (9 +3)
How many DNA molecules does a bacteria have, and where is it located
Bacteria have a single DNA molecule located in the cytoplasm (not surrounded by a nuclear envelope).
Which DNA has protein associated with it, bacterial or eukaryotic?
Eukaryotic chromosomes have protein
What are the small DNA circles in addition to the main bacterial DNA in bacteria
Plasmids, they replicate independently of the main DNA
Why are plasmids important to bacteria?
They have genes that confer antibiotic resistance
How do bacteria reproduce
binary fission (asexually)
T or F, the fusion of gametes occurs in bacteria?
F - they reproduce asexually
How is genetic material exchanged between bacteria: Name 3 ways
Transformation, Transduction, Conjugation
Fragments of DNA released by a broken cell are taken in by another cell
Transformation
A phage carries bacterial genes from one bacterial cell into another
Transduction
Two cells of different mating types come together, and genetic material is transferred from one to the other
Conjugation
This type of genetic exchange in bacteria involves contact between two cells
Conjugation
Conjugation has been studied the most in this type of bacteria
E. coli
These serve as conjugation bridges that pass from donor to recipient cell
F pili
MOst bacteria are heterotrophic or autotrophic?
Heterotrophic
______trophic must obtain organic compounds from other organisms (auto or hetero)
Hetero
What % of Bacteria and Archaebacteria are motile
50%
Chemical swtiches and connectors deciding which way it’s going to rotate so that a bacteria moves toward the source
Chemotaxis
Light controls the motion of the motor so that the bacteria either move toward it or away
Phototaxis
2 examples of microscopic photoautotrophs
anabaena (blue green algae that fixes nitrogen) and nostoc colonies
Bacteria that are able to manufacture their own organic molecules (hetero or autotrophic)
Autotrophs
Autotrophs that obtain their energy from light
photoautotrophs
Autotrophs that obtain energy by oxidizing inorganic chemicals (such as sulfur compounds likeH2S)
chemoautotrophs
Examples of chemoautotrophs
Mussels and crabs at geothermal vents in the ocean
Examples of macroscopic photoautotrophs
Photosynthetic organisms like plants
Saprobes and parasites are two types of what?) (chemoheterotrophs or photoheterotrophs)
Chemoheterotrophs
This type of bacteria gets their energy from dead organic matter. They are the majority of heterotrophic bacteria.
saprotrophs or saprobes
Lactobacillus and E. coli are examples of what?
saprobes (and chemoheterotrophs)
This type of chemohetrotroph only reproduces in living things
parasite
what are two examples of a parasite
Rickettsia (gram - pleomorphic bacteria, transmits disease by bites) and Chlamydia (spherical with no peptidoglycan in their cell walls, most common STDs)
Use light for making atp but cant get the job done without an organic carbon source
photoheterotrophs - very strange
two photoheterotrophs
Rhodospirillum and Rhodomicrobium
This type of heterotroph are Gram negative rods, important to the ecosystem because they convert nitrogen into ammonium that can then be used to make protein
Nitrogen fixers
An example of nitrogen fixer
Rhizobium, associated with bean plants
What does this describe:
1. anaerobic heterotrophs – 1st life forms
methanogens of archaea - modern example obligate anaerobes poisoned by O2 facultative can use or not use O2 2. Photoautotrophs developed as soup was depleted O2 as a toxin living examples of photoautotrophs 3. O2 metabolism evolves among O2 detoxifiers 1st among photoautotrophs -there are examples of O2 detoxifying anaerobes -all photosynthetic organisms are aerobes -most bacteria and extremophiles are -molecular evidence suggests many bacteria lost photosynthetic capabilities -fossil prokaryotes from 3.5 bil. yr.
Evolution and metabolic strategies
the Domain BActeria, Domain Archaea, and Domain Eukarya represent what?
Prokaryotic evolution based on genetic relationships - because the evidence from molecular biology has concluded that ancient prokaryotes split into two lineages early in the history of life. The modern descendants of these two ancient lines are the archaebacteria and the eubacteria
Which are more common, eubacteria or archaebacteria?
Eubacteria
Which inhabit very harsh environments more commonly, eubacteria or archaebacteria?
Archaebacteria
Methanogens, halobacteria, and thermoacidophiles are examples of what?
Archaebacteria
T or F, archaebacteria have peptidoglycan in their cell walls
F
Methanogens are the most common ____
archaebacteria
Methanogens are aerobic, T or F
F, they are strict anaerobes
rRNA sequences of archae are more similar to eukaryotes, T or F
T, some have histones, some do not
two examples of archaea
Crenoarchaeota – thermophiles
113 C, pH 2-3 at deep sea vent
Euryachaeota – halophiles and methanogens
5 clades of Bacteria (Eubacteria)
- Proteobacteria – gram (-) 5 subgroups, here are 2
a. Alpha -Rhizobium, Rickettsia, Agrobacterium, and the Protomitochondrion (bacteria that later was engulfed and became the mitochondrion)
b. gamma - Salmonella, E. coli - pathogens that tend to have endotoxins - Chlamydias – gram(-)
- spirochetes - Treponema, Borrelia
- Gram positive bacteria - Bacillus anthracis, Clostridium, Streptococcus pyogenes (strep throat), Staphylococcus aureus, soil actinomycetes, many of these are pathogens that tend to have exotoxins
- cyanobacteria - endosymbiont chloroplasts
Methanosarcina is an example of what type of bacteria? Eubacteria or Archaebacteria
Archaebacteria
Prokaryotic benefits
Nitrogen fixation and cycling nutrients in the soil, Antibiotic production,
Yogurt, pickles, olives, sauerkraut, cheese
Industrial compounds such as acetone,
Bioremediation
Are the following characteristics from a Gram positive or Gram Negative cell wall:
Thick peptidoglycan layer
Plasma membrane
gram positive
Is the following description form a Gram positive or Gram negative cell wall:
Outer membrane of polysaccharides and lipoprotein
Thin peptidoglycan
plasma membrane
Gram Negative
These disease causing poisons are secreted by Gram positive bacteria
exotoxin
These fever causing substances are produced by Gram negative bacteria
endotoxin
Unicellular or simple multicellular organisms that posses a eukaryotic cellular organization are called ————
Protists
The floating often microscopic organisms that are the base of food wabs in aquatic ecosystems are collectively called _______________
Plankton
Protista that ingest their food as animals do are informally called __________
Protozoa
This kingdom is the most diverse (most number of species)
Protista
This kingdom is polyphyletic (does not share a common ancestor)
Protista
amoebas move and obtain food by means of ______
pseudopodia
Foraminiferans secrete many-chambered shells called _____
tests
Some actinopods have long_______that protrude through pores in their skeletons
axopods
______ are unicellulare protozoa that are free-living or parasitic, move by means of flagella, and do not photosynthesize.
Flagellates
Paramecium and other —— move by means of cilia
ciliates
The ciliates oftern display a sexual phenomenon called ———-
conjugation
The ______ are a group of parasitic protozoa that form spores at some stage in their life
Sporozoans
_____ are algae charfacterized by two flagella, on wrapped around the center of the cell like a belt and the other projecting behind the cell
Dinoflagellates
A dinoflagellate bloom is known as a __________
Red tide
The _______ are photosynthetic protist with shells composed of two halves that fit together like a Petri dish
Diatoms
Chlorophyll a, Chlorophyll b, and carotenoids are found in green algae, _______, and plants
Euglenoids
the ______ ______ have pigmentation similare to the cyanobacteria
Green algae
Agar and carrageenan are economically improtant products derived from ______ _______
Red algae
The multicellular bodies of ____ _____ are differentiated into blades stipes, holdfasts and gas-filled floats
Brown algae
The feeding stage of plasmodial slime molds is a multinicleate ______
plasmodium
The ______ ______ ______ behave as unicellular organisms until reproduction, when the aggregate to form a slug
cellular slime molds
Water molds reproduce asexually by forming biflagellate ______, and sexually by forming ______
zoospores, oospores
Three types of protists showing how broad the diversity is
1. Diplomonads 2. Euglena - kinetoplastid Trypanosoma 3. Alveolata – subsurface cavities a. Dinoflagellates: corkscrew flagella b. Apicomplexans: Plasmodeium anopheles