Test 2 Flashcards

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1
Q

Standard Curve

A
  • Enzyme Kinetics
  • Vo vs Enzyme concentration graph
  • Vo = product concentration (nmol)/min
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2
Q

nmol

A

nmol = OD/m

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3
Q

Lineweaver-Burke Equation

A
  • y = mx + b

- (1/Vo) = (Km/Vmax)(1/[S]) + (1/Vmax)

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4
Q

Genotype Frequency

A

Number of people/Total number

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5
Q

Allele Frequency Homozygoud

A

[2(Number of People) + Heterozygous]/[2(Total number)]

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6
Q

pGLO

A

See picture

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7
Q

Microgram of Plasmid

A
  • Microgram of Plasmid = pGLO concentration (microgram/microliter) x volume added (microliter)
  • Unit: micrograms
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8
Q

Dilution Factor

A
  • Total Volume/Volume Spread

- Unitless

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9
Q

Transformation Efficiency

A
  • (# of colonies x dilution factor)/microgram plasmid

- Unit: # of colonies/microgram of plasmid

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