Test 1- Protein Quantification

Question 1

Slope, given A595 readings

Answer 1

m = (sum of xy)/(sum of x^2)

Question 2

Amount of protein

Answer 2

(Avg. OD of both samples)/m = mass (micrograms)

Question 3

Concentration of protein in the diluted sample

Answer 3

(mass in micrograms)/(vol. of diluted sample protein in microliters) = diluted concentration in micrograms/microliters

Question 4

Concentration of protein in the original sample

Answer 4

(diluted conc.) x (dilution factor) = original concentration

Question 5

Amount of protein present in the original sample

Answer 5

(original conc in mg/mL) x (original volume in mL) = original mass in mg